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D2291 Sigma-Aldrich

Pulse Marker 0.1-200 kb

for pulse-field DNA electrophoresis

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Properties

grade   for molecular biology
form   buffered aqueous glycerol solution
usage   25 uses
suitability   suitable for electrophoresis (pulsed-field DNA)
storage temp.   2-8°C

Description

Physical form

Solution in 10 mM Tris HCl, pH 8.0, 5 mM EDTA, 50% glycerol

Application

The 0.1 − 200 kb marker contains a mixture of 12 fragments consisting of Lambda DNA Hind III fragments plus uncut Lambda DNA and Lambda DNA concatemers. The recommended pulsed-field electrophoresis gel concentration is 1.0%. The uncut Lambda DNA monomer band (48.5 kb) has been adjusted to make it the brightest band. The recommended plug size is approximately 20 μl (0.5 μg) or two small graduations on the syringe.

Legal Information

Pulse Marker is a trademark of Sigma-Aldrich Co. LLC

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
1

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles
Peer-Reviewed Papers
15

References

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