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  • D2534 - cis-4,7,10,13,16,19-Docosahexaenoic acid

D2534 Sigma-Aldrich

cis-4,7,10,13,16,19-Docosahexaenoic acid


Synonym: DHA

  • CAS Number 6217-54-5

  • Empirical Formula (Hill Notation) C22H32O2

  • Molecular Weight 328.49

  •  Beilstein/REAXYS Number 1715505

  •  MDL number MFCD00065722

  •  PubChem Substance ID 24893696




1 g in ampule

25, 100 mg in ampule

Biochem/physiol Actions

Docosahexaenoic acid, DHA, is an omega-3 polyunsaturated fatty acid with 22 carbons and six double bonds, the first double bond occuring at position three from the methyl terminus (22:6 n-3). DHA is a component of lipid membranes and the myelin sheath. DHA also serves as a precursor for signaling molecules such as prostaglandins and eicosanoids.


Sealed ampule.

Safety & Documentation

Safety Information

NA 1993 / PGIII
WGK Germany 
Flash Point(F) 
143.6 °F
Flash Point(C) 
62 °C


Certificate of Analysis


Certificate of Origin


Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
IsProduct D2534, cis-4,7,10,13,16,19-Docosahexaenoic acid, a solution? If so, what is the solvent? If not, what is the density?
D2534 is not a solution; it is a neat oil. The density of D2534 is approximately 0.95 g/mL.
What is the best way to prepare a solution of Product D2534, cis-4,7,10,13,16,19-Docosahexaenoic acid?
D2534 is soluble in chloroform at a concentration of 50 mg/mL. For biological or biochemical applications, chloroform is probably not suitable. Information in our files indicates that the product would be expected to be soluble in 95% ethanol to at least 10 mg/mL.
After I prepare a solution ofProduct D2534, cis-4,7,10,13,16,19-Docosahexaenoic acid, in ethanol, how should it be stored?
The solution in ethanol would be unstable.It is best to make solutions up fresh.If this is not possible, divide the solution into aliquots and layer argon or nitrogen over the solution in each vial.Also, the product may be packaged in ampules, or kept in a vial with a cap plug or vacuum seal and stored at -70 °C.Any antioxidant such as BHT (di-tert-butyl hydroxytoluene) will aid in preventing oxidation of this product.BHT would be one of the better antioxidants to use.It can be used at 0.1% w/w or in the most extreme cases up to 1%.The most important thing to do to prevent oxidation is to exclude oxygen from contacting the product.
I know thatProduct D2534, cis-4,7,10,13,16,19-Docosahexaenoic acid, is very prone to oxidation. I would like to study the oxidation in detail. Do you have any references?
A rapid method for studying the oxidation of fatty acids, including linoleic acid, eicosapentaenoic acid and docosahexaenoic acid, has been published. Boyd, L.C., et al., A rapid method for determining the oxidation of n-3 fatty acids. J. Am. Oil Chem. Soc., 69, 325-330 (1992).
Are all of the double bonds in theProduct D2534, cis-4,7,10,13,16,19-Docosahexaenoic acid, in the cis-configuration?
Yes, this product's double bond configuration is cis.
How do I find price and availability?
There are several ways to find pricing and availability for our products.Once you log onto our website, you will find the price and availability displayed on the product detail page.You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 orview local office numbers.
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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