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D4902 Sigma-Aldrich

Dexamethasone

powder, BioReagent, suitable for cell culture, ≥97%

Synonym: (11β,16α)-9-Fluoro-11,17,21-trihydroxy-16-methylpregna-1,4-diene-3,20-dione, 9α-Fluoro-16α-methyl-11β,17α,21-trihydroxy-1,4-pregnadiene-3,20-dione, 9α-Fluoro-16α-methylprednisolone, Prednisolone F

  • CAS Number 50-02-2

  • Empirical Formula (Hill Notation) C22H29FO5

  • Molecular Weight 392.46

  •  Beilstein/REAXYS Number 2066651

  •  EC Number 200-003-9

  •  MDL number MFCD00064136

  •  PubChem Substance ID 24893906

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Properties

Related Categories Bioactives and Supplements for Stem Cell Expansion, Bioactives/Supplements, Cell Biology, Cell Culture, Cell Signaling and Neuroscience,
InChI Key   UREBDLICKHMUKA-CXSFZGCWSA-N
product line   BioReagent
assay   ≥97%
form   powder
potency   4-500 ng/mL
mp   262-264 °C (lit.)
solubility   methanol: 25 mg/mL
suitability   suitable for cell culture
storage temp.   2-8°C
Gene Information   human ... ABCB1(5243), CYP3A4(1576), IL4(3565), IL5(3567), NR3C1(2908)
mouse ... Abcb1a(18671), Abcb1b(18669), Ifng(15978), Nos2(18126), Ptgs2(19225), Tnf(21926)
rat ... Ar(24208), Nr3c1(24413), Tnf(24835)

Description

Application

Dexamethasone is used to study apoptosis, cell signaling pathways, cell differentiation and gene expression.

Packaging

1 g in glass bottle

25, 100, 500 mg in glass bottle

Biochem/physiol Actions

Glucocorticoid anti-inflammatory agent. Regulates T cell survival, growth, and differentiation. Inhibits the induction of nitric oxide synthase.

It is poorly metabolized in the body and has great affinity and bioavailability.

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

Physical form

powder 2-8 °C; stock-frozen in working aliquots, avoid repeated freeze/thaw

Reconstitution

To prepare 20 μg/ml stock solution, add 1ml absolute ethanol per mg product; gently swirl to dissolve. Add 49 ml sterile medium per ml of ethanol added, while mixing, to acheive final concentration of 20 μg/ml.

Safety & Documentation

Safety Information

Symbol 
GHS08  GHS08
Signal word 
Danger
Hazard statements 
Precautionary statements 
Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
2
RTECS 
TU3980000

Documents

Certificate of Analysis

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Certificate of Origin

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Frequently Asked Questions

What is Product D4902, Dexamethasone, soluble in?
Dexamethasone is practically insoluble in water. It is soluble in ethanol, methanol, acetone, dioxane and slightly soluble in chloroform.
How should I reconstitute Product D4902, Dexamethasone, for tissue culture use?
The tissue culture grade product isdissolved in ethanol at a concentration of 1 mg/ml forming a clear, colorless solution. It is also possible to prepare a stock solution of dexamethasone in DMSO at 1.5 mM.
Do you have references for the use of Product D4902, Dexamethasone?
References can be found on the product detail page:Migliorati, G., et al., Dexamethasone and interleukins modulate apoptosis of murine thymocytes and peripheral T-lymphocytes. Pharmacol Res., 30(1), 43-52 (1994).Bansal, N., et al., Apoptosis: mode of cell death induced in T cell leukemia lines by dexamethasone and other agents. FASEB J. 5(2), 211-6 (1991).Shiao, R.T., et al., Dexamethasone and suramin inhibit cell proliferation and interleukin-6-mediated immunoglobulin secretion in human lymphoid and multiple myeloma cell lines. Leuk. Lymphoma, 17(5-6), 485-494 (1995).Migliorati, G., et al, Dexamethasone induces apoptosis in mouse natural killer cells and cytotoxic T lymphocytes. Immunology, 81(1), 21-26 (1994).
How long is Product D4902, Dexamethasone, stable in solution?
Dexamethasone solutions are stable for at least 30 days when stored at 4 °C and protected from light. The solution may be aliquoted and stored at -20 °C. However, insufficient information is available to assess the shelf-life of the frozen solution.
What cell lines has Product D4902, Dexamethasone, been tested on?
The cell lines on which this product is tested can vary from lot to lot. Some typically used cell lines are 3T3 fibroblasts, BHK, L929 and CHO.
What is the shelf life of Product D4902, Dexamethasone?
The product has a 5 year shelf life.
Product D4902, Dexamethasone is not water soluble. Do you have a water soluble Dexamethasone?
Product D2915 is water soluble.
Can I use Product D4902, Dexamethasone, to inject into mice?
We do not perform animal studies with our products, but it can be used in cell culture (D2915 - cell culture grade version, or USP grade product D9184).
Are Products D4902 and D2915, sterile?
No. This product is not sterile, but cell culture testing did not find the product to be toxic to cells.
Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
How do I find price and availability?
There are several ways to find pricing and availability for our products.Once you log onto our website, you will find the price and availability displayed on the product detail page.You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 orview local office numbers.
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
My question is not addressed here, how can I contact Technical Service for assistance?
Ask a Scientist here.
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Peer-Reviewed Papers
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References

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