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D8783 Sigma-Aldrich

7-Deaza-2′-deoxyguanosine 5′-triphosphate lithium salt

10 mM in H2O

Synonym: −N7-dGTP, 7-Deaza-dGTP

  • CAS Number 101515-08-6

  • Empirical Formula (Hill Notation) C11H17N4O13P3

  • Molecular Weight 506.19

  •  MDL number MFCD00171614

  •  PubChem Substance ID 24894227

  •  NACRES NA.52

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Properties

Related Categories Biochemicals and Reagents, Nucleosides, Nucleotides, Oligonucleotides, Nucleotides More...
form   liquid
concentration   10 mM in H2O
color   colorless
shipped in   dry ice
storage temp.   −20°C
SMILES string   NC1=NC(=O)c2ccn(C3CC(O)C(COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O3)c2N1
InChI   1S/C11H17N4O13P3/c12-11-13-9-5(10(17)14-11)1-2-15(9)8-3-6(16)7(26-8)4-25-30(21,22)28-31(23,24)27-29(18,19)20/h1-2,6-8,16H,3-4H2,(H,21,22)(H,23,24)(H2,18,19,20)(H3,12,13,14,17)
InChI key   DLLXAZJTLIUPAI-UHFFFAOYSA-N

Description

General description

7-Deaza-2′-deoxyguanosine 5′-triphosphate enhances polymerase chain reaction (PCR) product yield.

Application

G residues can form secondary structures which are not fully denatured during electrophoresis. This can lead to sequence ambiguities. 7-Deaza-G doesn′t form these secondary structures, so avoids sequence ambiguities.

Biochem/physiol Actions

This dGTP analog pairs weakly with conventional bases to minimize DNA secondary structures, while being a good substrate for DNA ploymerases.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
2

Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Protocols & Articles
Peer-Reviewed Papers
15

References

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