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D9538 Sigma-Aldrich

N,N′-(1,2-Dihydroxyethylene)bis-acrylamide

for electrophoresis

Synonym: DHEBA, N,N′-Bisacryloyl-1,2-dihydroxy-1,2-ethylenediamine

  • CAS Number 868-63-3

  • Empirical Formula (Hill Notation) C8H12N2O4

  • Molecular Weight 200.19

  •  Beilstein/REAXYS Number 1941875

  •  EC Number 212-780-1

  •  MDL number MFCD00008624

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Properties

grade   for electrophoresis
conductivity   <60 μmho (2% solution)
storage temp.   2-8°C
SMILES string   OC(NC(=O)C=C)C(O)NC(=O)C=C
InChI   1S/C8H12N2O4/c1-3-5(11)9-7(13)8(14)10-6(12)4-2/h3-4,7-8,13-14H,1-2H2,(H,9,11)(H,10,12)
InChI key   ZMLXKXHICXTSDM-UHFFFAOYSA-N

Description

Application

Reversible cross-linker for polyacrylamide gels that produces a gel, which is soluble in dilute (2%) periodic acid at room temperature for 1-2 hr. Gel solubilization is useful when recovering protein from polyacrylamide gels.

Analysis Note

Tested for use as a cross-linking agent for recovery of separated biomolecules.

Safety & Documentation

Safety Information

Symbol 
GHS07  GHS07
Signal word 
Warning
Hazard statements 
Precautionary statements 
Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis (COA)

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Protocols & Articles
Peer-Reviewed Papers
15

References

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