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DNB100 Sigma-Aldrich

GenElute Soil DNA Isolation Kit

Green Alternative

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Properties

Related Categories 12 Principles Aligned Products, Chemical Synthesis, DNA & RNA Purification, Genomic DNA Purification, Greener Alternative Products,
greener alternative product characteristics   Designing Safer Chemicals
Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.
shipped in   ambient
storage temp.   room temp

Description

Other Notes

Sigma Life Science is committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has Inherently Safer Chemistry, compared to the standard use of phenol and chloroform to perform DNA extractions.

Unit Definition

Preps

Application

• Quantitative, real-time PCR
• Conventional PCR
• Southern Blot Analysis
GenElute Soil DNA Isolation Kit has been used to extract microbial DNA from anodic biofilms of microbial fuel cells.

Features and Benefits

• Process all types of soil - All types of soil samples can be processed with this kit, including common soil samples such as clay, loam and sandy soil as well as difficult soil samples with high humic acid content such as compost and manure.
• Remove all humic acid from DNA samples - The kit removes all traces of humic acid using the provided Humic Acid Removal Columns and the OSR Solution
• Rapid detection of microorganisms in soil samples - Isolate total DNA from all microorganisms found in soil, including bacteria, fungi and algae.
• Fast and easy processing - Rapid spin column format allows for the isolation of DNA in under 30 minutes.
• Isolate high quality total DNA - The purified DNA is free from all inhibitors including humic acid, and ca be used directly in downstream applications including PCR.

General description

Sigma’s GenElute Soil DNA Isolation Kit provides a convenient and rapid method for the detection of microorganisms from soil samples. All types of soil samples can be processed with this kit, including common soil samples and difficult soil samples with high humic acid content such as compost and manure. The kit removes all traces of humic acid using the provided Humic Acid Removal Column and the OSR (Organic Substance Removal) Solution. A simple and rapid spin column procedure is then used to further purify the DNA. Total genomic DNA can be isolated and purified from all the various microorganisms found in soil, such as bacteria, fungi and algae. The purified DNA is of the highest quality and is fully compatible with downstream PCR applications, as all humic acid substances and PCR inhibitors are removed during the isolation.

• Isolate total DNA from all microorganisms found in soil, including bacteria, fungi and algae
• Process all types of soil, including common soil samples and difficult samples with high humic acid
• Remove all humic acids using the provided Humic Acid Removal Columns and the OSR Solution
• Isolated DNA is free of inhibitors such as humic acid - ready for PCR applications
• Easy- to-use beads for extraction - fast without any time consuming sample grinding

Legal Information

GenElute is a trademark of Sigma-Aldrich Co. LLC

Safety & Documentation

Safety Information

RIDADR 
UN 2810 6.1 / PGIII

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Protocols & Articles
Peer-Reviewed Papers
15

References

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