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DNB300 Sigma-Aldrich

GenElute Urine Cell-Free DNA Purification Mini Kit

Green Alternative

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Related Categories 12 Principles Aligned Products, Chemical Synthesis, DNA & RNA Purification, Genomic DNA Purification, Greener Alternative Products,
greener alternative product characteristics   Designing Safer Chemicals
Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.
shipped in   ambient
storage temp.   room temp

Description

Other Notes

Sigma Life Science is committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has Inherently Safer Chemistry, compared to the standard use of phenol and chloroform to perform DNA extractions.

Unit Definition

Preps

Application

• PCR
• qPCR
• Southern blot
• Methylation-sensitive PCR
• CpG array
• Restriction enzyme digestions
• Viral detection
• Bacterial detection
• Microarray
• NGS

General description

Sigma’s GenElute Urine Cell-Free DNA Purification Mini Kit provides a fast, reliable and simple procedures for isolating cell-free circulating DNA (cfc-DNA) from various amounts of urine ranging from 250 μL up to 2 mL. Purification is based on spin column chromatography that uses Sigma’s proprietary resin separation matrix. The kits are designed to isolate all sizes of cfc-DNA from either fresh or frozen urine samples. Moreover, these kits allow the user to elute the purified cfc-DNA into a flexible elution volume ranging from 25 μL to 50 μL. The purified urine cfc-DNA is eluted in an elution buffer that is compatible with all downstream applications including PCR, qPCR, methylation-sensitive PCR and Southern Blot analysis, and NGS.

• Isolate all sizes of circulating DNA from fresh, preserved or frozen urine samples
• Isolate viral DNA
• Versatile urine input volumes (250 μL - 2 mL)
• Concentrate circulating DNA into a flexible elution volume ranging from (50 μL - 100 μL)
• Isolate inhibitor-free cell-free circulating DNA
• Purify high-quality DNA in 15-20 minutes

Legal Information

GenElute is a trademark of Sigma-Aldrich Co. LLC

Safety & Documentation

Safety Information

RIDADR 
UN 3265 8 / PGIII

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Protocols & Articles
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