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E6758 Sigma-Aldrich

Ethylenediaminetetraacetic acid

anhydrous, crystalline, BioReagent, suitable for cell culture

Synonym: (Ethylenedinitrilo)tetraacetic acid, EDTA, Edathamil, Ethylenedinitrilotetraacetic acid

  • CAS Number 60-00-4

  • Linear Formula (HO2CCH2)2NCH2CH2N(CH2CO2H)2

  • Molecular Weight 292.24

  •  Beilstein/REAXYS Number 1716295

  •  EC Number 200-449-4

  •  MDL number MFCD00003541

  •  eCl@ss 39030907

  •  PubChem Substance ID 24894634

  •  NACRES NA.25

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Description

Application

Ethylenediaminetetraacetic acid has been used:
• as a component of peptide dilution buffer for intracellular BH3 (Bcl-2 homology (BH) domain) profiling and validation
• as a medium component in the hepatic stellate cell (HSCs) culture
• as a component of homogenization buffer for protein extraction from eye tissues
• for stopping benzonase reaction in lysis buffer

Packaging

100, 500 g in poly bottle

Biochem/physiol Actions

Ethylenediaminetetraacetic acid (EDTA) is an anticoagulant, majorly used in preventing clotting of blood samples. EDTA may trigger aggregation of platelets resulting in pseudothrombocytopenia. EDTA along with tetracycline imparts serves as root conditioning agent and provides relief in gum infection. EDTA-tetracycline therapy decreases coronary artery calcium levels in atherosclerosis. Calcium Disodium EDTA administration is effective in slowing down the progression of chronic kidney disease.

Safety & Documentation

Safety Information

Symbol 
GHS07  GHS07
Signal word 
Warning
Hazard statements 
Precautionary statements 
Personal Protective Equipment 
RIDADR 
UN 3077 9 / PGIII
WGK Germany 
2
RTECS 
AH4025000

Single- & multi-channel pipettes from BRAND

Pipette Controller from Brand
Protocols & Articles

Articles

Creating Transgenic Mice using CRISPR-Cas9 Genome Editing

While several genome editing tools have been developed in recent years, including zinc finger-based strategies and TALENs (transcription activator-like effector nucleases), none have been as efficien...
Keywords: Amplification, Cell culture, Enzyme-linked immunosorbent assay, Gene expression, Genetic, Genetics, Immunohistochemistry, Immunology, Nutrition, Polymerase chain reaction, Recombination, Southern blot, Transcription, Transfection, Western blot

Protocols

Restriction Enzyme Cloning Manual Buffer Recipes

Tip 1: DNA is quite stable in TE buffer at 4ºC. If stored in elution buffer or water then freezing at -20 ºC is advised.
Keywords: Phase transitions, Sequencing, Sterilizations

Selecting Correctly Expressing Recombinants

Blue / White colony screening is a strategy to quickly and easily distinguish between recombinant and non-recombinant colonies. It requires a special vector and a special strain of E. coli. It is par...
Keywords: AGE, Amplification, Antibiotics, Cell disruption, Centrifugation, Cloning, Degradations, Detergents, Diagnostic, Digestions, Electrophoresis, Gel electrophoresis, Molecular biology, Peptide synthesis, Phase transitions, Polymerase chain reaction, Precipitation, Purification, Sequencing, Transfection, transformation

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Cell Culture Troubleshooting Guide

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Peer-Reviewed Papers
15

References

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