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E6783 Sigma-Aldrich

pFLAG-CMV-3 Expression Vector

shuttle vector for transient or stable expression of N-terminal FLAG

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Properties

Related Categories Cloning and Expression, For Stable Expression, Mammalian Expression Vectors, Molecular Biology, Sigma Expression Vectors More...
grade   for molecular biology
form   buffered aqueous solution
conjugate   FLAG® tagged
Peptide tag location   N-terminal
shipped in   dry ice
storage temp.   −20°C

Description

Components

• pFLAG-CMV-3 Expression Vector 20 μg (E8770) is supplied as 0.5 mg/ml in 10 mM Tris-HCl (pH 8.0) with 1 mM EDTA.
• pFLAG-CMV-3-BAP Control Plasmid 20 μg (C3972) is supplied as 0.5 mg/ml in 10 mM Tris-HCl (pH 8.0) with 1 mM EDTA.

General description

The pFLAG-CMV-3 Expression Vector is a 6.3 kb vector used for transient or stable expression in
mammalian cells. The vector is a derivative of pCMV5 used for transient or stable expression and secretion of a properly inserted open reading frame as an N-terminal FLAG fusion protein.

The pFLAG-CMV-3 Expression Vector is a shuttle vector containing both bacterial and SV40 origins of replication for propagation in both E. coli and mammalian cells. Efficiency of replication and genomic integration is optimal when using host cells that express the SV40 large T antigen.

The pFLAG-CMV-3-BAP Control Plasmid is a 7.7 kb derivative of pCMV51 used for transient expression and secretion of N-terminal FLAG bacterial alkaline phosphatase fusion protein in mammalian cells.

The FLAG® epitope is a small, hydrophilic 8 amino acid tag (DYKDDDDK) that provides for sensitive detection and high quality purification using ANTI-FLAG products available from Sigma-Aldrich.

Vector Maps and Sequences

Principle

The promoter-regulatory region of the human cytomegalovirus drives transcription of FLAG-fusion constructs. The preprotrypsin leader sequence precedes the FLAG® sequence and directs secretion of the fusion protein into the culture medium. The aminoglycoside phosphotransferase II gene (neo-r) confers resistance to aminoglycosides such as G 418, allowing for selection of stable transfectants.

Legal Information

FLAG is a registered trademark of Sigma-Aldrich Co. LLC

pFLAG-CMV is a trademark of Sigma-Aldrich Co. LLC

pFLAG-CMV3 is a trademark of Sigma-Aldrich Co. LLC

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
Protocols & Articles

Articles

Blue-White Screening & Protocols for Colony Selection

Blue-white screening is a rapid and efficient technique for the identification of recombinant bacteria. It relies on the activity of β-galactosidase, an enzyme occurring in E. coli, which cleaves lac...
Keywords: Antibiotics, Cloning, Culture media, Gene expression, Immunocytochemistry, Molecular biology, Peptide synthesis, Polymerase chain reaction, Recombination, Sterilizations, transformation

pSF-FLAG® Vectors from Oxford Genetics

The classic FLAG and 3xFLAG expression vectors have been converted to SnapFast™ Expression Vectors – combining two highly functional systems into one product. Additional information about the SnapFas...
Keywords: Flow cytometry, Gene expression, Immunocytochemistry, Immunoprecipitation, Purification, Western blot

Peer-Reviewed Papers
15

References

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