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E6908 Sigma-Aldrich

pFLAG-CMV-5.1 Expression Vector

shuttle vector for intracellular transient expression of C-terminal FLAG

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Properties

Related Categories Cloning and Expression, For Transient Expression, Mammalian Expression Vectors, Molecular Biology, Sigma Expression Vectors More...
grade   for molecular biology
form   buffered aqueous solution
conjugate   FLAG® tagged
Peptide tag location   C-terminal
shipped in   dry ice
storage temp.   −20°C

Description

Components

• pFLAG-CMV-5.1 Expression Vector 20 μg (E7901) is supplied as 0.5 mg/ml in 10 mM Tris-HCl (pH 8.0) with 1 mM EDTA.
• pFLAG-CMV-5b-BAP Control Plasmid 20 μg (P5225) is supplied as 0.5 mg/ml in 10 mM Tris-HCl (pH 8.0) with 1 mM EDTA.

General description

The pFLAG-CMV-5.1 Expression Vector is a 4.7 kb derivative of the pCMV5 transient expression vector, used for establishing transient intracellular expression of C-terminal FLAG® fusion proteins in mammalian cells.

pFLAG-CMV-5.1 is a shuttle vector for E. coli and mammalian cells. Efficiency of replication and genomic integration is optimal when using an SV40 T antigenexpressing host.

The pFLAG-CMV-5b-BAP Control Plasmid is a 6.0 kb derivative of the pCMV5 transient expression vector for intracellular expression of C-terminal FLAG bacterial alkaline phosphatase fusion protein in mammalian cells.

Vector Maps and Sequences

Principle

The promoter-regulatory region of the human cytomegalovirus drives transcription of [TM="FLAG′}-fusion constructs. The multiple cloning region of the [TM="pFLAG-CMV′}- 5.1 vector is not preceded by a translational initiation signal. A translational start sequence must be included with the insert DNA. The multiple cloning region of the pFLAG-CMV-5.1 vector is compatible with other CMV vectors.

Legal Information

FLAG is a registered trademark of Sigma-Aldrich Co. LLC

pFLAG-CMV is a trademark of Sigma-Aldrich Co. LLC

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
Protocols & Articles

Articles

Introduction to Blue-White Screening

Blue-white screening is a rapid and efficient technique for the identification of recombinant bacteria. It relies on the activity of β-galactosidase, an enzyme occurring in E. coli, which cleaves lac...
Keywords: Antibiotics, Cloning, Culture media, Gene expression, Immunocytochemistry, Peptide synthesis, Polymerase chain reaction, Recombination, Sterilizations, transformation

pSF-FLAG® Vectors from Oxford Genetics

The classic FLAG and 3xFLAG expression vectors have been converted to SnapFast™ Expression Vectors – combining two highly functional systems into one product. Additional information about the SnapFas...
Keywords: Flow cytometry, Gene expression, Immunocytochemistry, Immunoprecipitation, Purification, Western blot

Peer-Reviewed Papers
15

References

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