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E8008 Sigma-Aldrich

Ethylenediaminetetraacetic acid solution

0.02% in DPBS (0.5 mM), sterile-filtered, BioReagent, suitable for cell culture

Synonym: EDTA solution

  • CAS Number 60-00-4

  • Empirical Formula (Hill Notation) C10H16N2O8

  • Molecular Weight 292.24

  •  Beilstein/REAXYS Number 1716295

  •  MDL number MFCD00003541

  •  eCl@ss 39030907

  •  PubChem Substance ID 24894664

  •  NACRES NA.75

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Properties

Related Categories Biochemicals and Reagents, Calcium Signaling Research Tools, Cell Biology, Cell Culture, Cell Dissociation,
Quality Level   200
sterility   sterile-filtered
product line   BioReagent
form   solution
concentration   0.02% in DPBS (0.5 mM)
application(s)   cell culture | mammalian: suitable
impurities   endotoxin, tested
pH   7.1-7.7
shipped in   wet ice
storage temp.   2-8°C
SMILES string   OC(=O)CN(CCN(CC(O)=O)CC(O)=O)CC(O)=O
InChI   1S/C10H16N2O8/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20)
InChI key   KCXVZYZYPLLWCC-UHFFFAOYSA-N

Description

General description

Ethylenediaminetetraacetic acid (EDTA) is an aminopolycarboxylic acid and a hexadentate ligand. It chelates with metal ions to form an octahedral complex especially with cations. Ethylenediaminetetraacetic acid (EDTA) is an anticoagulant, which prevents clotting of blood. It is implicated in platelet aggregation in pseudothrombocytopenia. EDTA chelates with calcium and is effective in solubilisation of the atheromatous plaques. Calcium disodium EDTA administration is effective in slowing down the progression of chronic kidney disease. It is a chelator used to remove calcium from cell washing and suspension media. EDTA reduces cell clumping.

Application

Ethylenediaminetetraacetic acid solution has been used to remove confluent cell monolayers in swine testicular (ST-cells) culture. It has also been used to detach embryonic stem cells (ESCs) from matrigel and primary human fibroblasts.

Physical form

Solution in Dulbecco′s phosphate buffered saline without calcium and magnesium

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
nwg
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Protocols & Articles

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Peer-Reviewed Papers
15

References

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