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  • E8145 - Ethylene glycol-bis(β-aminoethyl ether)-N,N,N′,N′-tetraacetic acid tetrasodium salt

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E8145 Sigma-Aldrich

Ethylene glycol-bis(β-aminoethyl ether)-N,N,N′,N′-tetraacetic acid tetrasodium salt

≥97%

Synonym: EGTA tetrasodium, Egtazic acid tetrasodium, Ethylene-bis(oxyethylenenitrilo)tetraacetic acid tetrasodium

  • CAS Number 13368-13-3

  • Empirical Formula (Hill Notation) C14H20N2O10Na4

  • Molecular Weight 468.28

  •  MDL number MFCD00274420

  •  PubChem Substance ID 24894667

  •  NACRES NA.77

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Properties

Related Categories Calcium Signaling Research Tools, Cell Biology, Cell Signaling and Neuroscience, Intracellular Calcium Signaling, Metal binding chelators More...
InChI Key   BAOGCDPNNOBWQZ-UHFFFAOYSA-J
Quality Level   200
assay   ≥97%
solubility   H2O: soluble 10%, clear, colorless

Description

Application

A chelating agent useful for the determination of calcium in the presence of magnesium. Repeated washing with EGTA-buffer can experimentally deplete cultured cells of Ca2+.

EGTA is suitable for use:
• in media for chelating Ca2+ to facilitate efficient incorporation of Sr2+ into the oocytes
• as a component of phosphatase wash buffer used to wash magnetic beads during dephosphorylation and radiolabeling of RNA segments cross-linked to immunoprecipitated proteins
• as a component of oocyte activation media

Packaging

10, 50 g in glass bottle

Biochem/physiol Actions

Ethylene glycol-bis(ß-aminoethyl ether)-N,N,N,N-tetra acetic acid tetrasodium salt (EGTA) is a calcium chelator that binds to Ca2+ in the presence of Mg2+ at a ratio of 1:1.

Ethylene glycol-bis(β-aminoethyl ether)-N,N,N′,N′-tetra acetic acid tetrasodium salt (EGTA) dissociates mucin complex and is useful in immunohistochemical studies. It suppresses root growth and mitotic index in germinating wheat seeds. It is used as a decalifying agent in root canal biomechanical preparation and is effective compared to EDTA and cyclohexanediaminetetraacetic acid (CDTA).

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Protocols & Articles
Peer-Reviewed Papers
15

References

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