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EMS0001 Sigma-Aldrich

PNGase Fast

recombinant, expressed in E. coli

Synonym: N-Glycosidase F, PNGase F, Peptide N-glycosidase

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Properties

recombinant   expressed in E. coli
form   liquid
shipped in   wet ice
storage temp.   2-8°C

Description

General description

PNGase Fast enables complete and rapid deglycosylation of antibodies and immunoglobulin fusion proteins, as well as other glycoproteins, to be prepared for downstream chromatography or mass spectrometry analysis. PNGase Fast creates an optimized workflow, reducing processing time without compromising sensitivity or reproducibility.

PNGase Fast is proteomic grade PNGase F combined with a buffer able to yield results similar to a conventional 20-hour protocol with overnight digest while reducing workflow time to about 1 hour with a 15 minute digest. Substrate reduction prior to PNGase Fast treatment is optional, but required for complete deglycosylation of some samples. PNGase Fast buffer and enzyme can be used instead of conventional filter-assisted workflows to allow for comparable results with significant time savings.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
Protocols & Articles

Articles

A Rapid, Streamlined Workflow for Glycan Sample Preparation and Analysis by LC-MSpngase fast asms

Comparison of PNGase F workflows for glycan release. The traditional workflow (top) involves multiple wash steps and an overnight digestion. With PNGase Fast (bottom), digestion is complete in 15 min...
Judy Boland, Amber Henry, Nicolas Caffarelli, Gordon Nicol, Jeffrey L. Turner, and Kevin Ray
Keywords: Aminations, Detergents, Digestions, Electrophoresis, High performance liquid chromatography, Mass spectrometry, PAGE, Reductions, Reductive aminations

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