EMAIL THIS PAGE TO A FRIEND
EMS0004 Sigma-Aldrich

SOLu-Trypsin

recombinant, expressed in Pichia pastoris, Proteomics Grade, liquid

Synonym: Mass Spectrometry Trypsin

Purchase

Description

Application

Poster: Evaluation of Recombinant, Chemically Treated Trypsin in Proteomics and Protein Characterization Assays

Features and Benefits

• Ready to use - no preparation, such as reconstitution or thawing, is required
• Fits seamlessly into established workflow - no need to modify protocols
• Eliminates waste - remains stable in the refrigerator after use so there is no need to discard excess product
• Recombinant, porcine sequence - no chymotryptic activity
• Stable for short-term use at room temperature in an autosampler or on a liquid handling robot

General description

SOLu-Trypsin (EMS0004) is our exclusive, Advanced Proteomics Grade enzyme that is solution stable for mass spectrometry. Designed to be stable in solution when refrigerated, SOLu-Trypsin can be used immediately without preparation. Other forms of trypsin require thawing or reconstitution, and must be discarded if not used immediately. SOLu-Trypsin allows excess product to be saved for future use, thus eliminating unnecessary waste and cost. SOLu-Trypsin is formulated with a high-purity recombinant trypsin, free of chymotryptic activity, to ensure high fidelity digestion.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
Protocols & Articles

Articles

Development of a Simple and Rapid Digestion Protocol for Proteomics Sample Preparation

Reliable and reproducible results in mass spectrometry proteomics analyses are highly dependent on robust sample preparation. Sample preparation workflows are often cumbersome and generally involve c...
Zhiyun Cao, Amber Henry, Judy Boland, Nicolas Caffarelli, Jeffrey Turner, Kevin Ray
Keywords: Alkylations, Cell disruption, Detergents, Digestions, Mass spectrometry, Nucleic acid denaturation, Proteomics, Reductions, Sample preparations

Evaluation of Recombinant, Chemically Treated Trypsin in Proteomics and Protein Characterization Assays

Human protein extract was digested using native and recombinant trypsins in a traditional overnight 37°C digest and a rapid 2 hr 45°C protocol. In all cases, similar numbers of proteins were identifi...
Judy Boland, Nicolas Caffarelli, Gordon Nicol, Amber Henry, Jeffrey L. Turner, and Kevin Ray
MilliporeSigma, A business of Merck KGaA, Darmstadt, Germany, St. Louis MO 63103
Keywords: Mass spectrometry, Proteomics, Sequencing

UHPLC Analysis of Deglycosylated SigmaMAb Tryptic Digest on BIOshell A160 Peptide C18

From our library of Articles, Sigma-Aldrich presents UHPLC Analysis of Deglycosylated SigmaMAb Tryptic Digest on BIOshell A160 Peptide C18

Related Content

Enzymes & Proteins

Application Index | Enzyme Index | Substrate Index | Inhibitor Index | Cofactor Index | Lectin Index
Keywords: Cell signaling, Diagnostic, Drug discovery, Molecular biology

Related Products

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?