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EMS0005 Sigma-Aldrich

SOLu-Trypsin Dimethylated

Dimethlyated, Proteomics Grade, recombinant, expressed in Pichia pastoris, liquid

Synonym: Mass Spectrometry Trypsin Dimethlyated

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Description

General description

SOLu-Trypsin Dimethylated (EMS0005) is our exclusive, Advanced Proteomics Grade enzyme that is solution stable for mass spectrometry. Designed to be stable in solution when refrigerated, SOLu-Trypsin Dimethylated can be used immediately without preparation. Other forms of trypsin require thawing or reconstitution, and must be discarded if not used immediately. SOLu-Trypsin Dimethylated allows excess product to be saved for future use, thus eliminating unnecessary waste and cost. SOLu-Trypsin Dimethylated is formulated with a high-purity recombinant trypsin, free of chymotryptic activity, to ensure high fidelity digestion.

Features and Benefits

• Ready to use - no preparation, such as reconstitution or thawing, is required
• Fits seamlessly into established workflow - no need to modify protocols
• Eliminates waste - remains stable in the refrigerator after use so there is no need to discard excess product
• Recombinant, porcine sequence - no chymotryptic activity
• Stable for short-term use at room temperature in an autosampler or on a liquid handling robot

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 1
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Protocols & Articles

Articles

Evaluation of Recombinant, Chemically Treated Trypsin in Proteomics and Protein Characterization Assays

Human protein extract was digested using native and recombinant trypsins in a traditional overnight 37°C digest and a rapid 2 hr 45°C protocol. In all cases, similar numbers of proteins were identifi...
Judy Boland, Nicolas Caffarelli, Gordon Nicol, Amber Henry, Jeffrey L. Turner, and Kevin Ray
MilliporeSigma, A business of Merck KGaA, Darmstadt, Germany, St. Louis MO 63103
Keywords: Mass spectrometry, Proteomics, Sequencing

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