• USA Home
  • EPI021 - AHCYActivity Fluorometric Assay Kit

EMAIL THIS PAGE TO A FRIEND
EPI021 Sigma-Aldrich

AHCYActivity Fluorometric Assay Kit

sufficient for 100 fluorometric tests

  •  NACRES NA.84

Purchase

Description

General description

Adenosylhomocysteinase (AHCY) (EC 3.3.1.1) or S-adenosylhomocysteine hydrolase (SAHH); is an enzyme that catalyzes the reversible hydrolysis of S-adenosyl homocysteine (SAH) to adenosine and homocysteine. AHCY regulates the intracellular SAH concentration which in turn regulates S-adenosyl methionine (SAM)-dependent methyltransferases. Down-regulation of AHCY has been associated with certain forms of cancer and Huntington’s disease, while in Wilson’s disease; the enzyme is inhibited by the accumulated copper. Mutations in the AHCY gene cause SAHH deficiency disease.

Suitability

Suitable for the detection of Adenosylhomocysteinase (AHCY) activty in purified recombinant protein or cell and tissue lysate.

Principle

The AHCY Activity Assay kit can kinetically measure AHCY activity by detecting adenosine generation resulting from the hydrolysis of SAH. Adenosine is detected via a multi-step reaction, resulting in the generation of an intermediate that reacts with the probe. The fluorescent product is measured at. limit of quantification (LOQ) is 1 U of recombinant human AHCY.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis (COA)

Please Enter a Lot Number
Protocols & Articles
Related Products

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?