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F3651 Sigma-Aldrich

Fluorescein 5(6)-isothiocyanate

≥90% (HPLC)

Synonym: FITC, Fluorescein isothiocyanate

  • CAS Number 27072-45-3

  • Empirical Formula (Hill Notation) C21H11NO5S

  • Molecular Weight 389.38

  •  Beilstein/REAXYS Number 1407295

  •  EC Number 248-207-7

  •  MDL number MFCD00005063

  •  PubChem Substance ID 24894855

  •  NACRES NA.47

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Description

General description

Fluorescein isothiocyanate (FITC) is widely used to attach a fluorescent label to proteins via the amine group. The isothiocyanate group reacts with amino terminal and primary amines in proteins. It has been used for the labeling of proteins including antibodies and lectins.

Application

Fluorescein 5(6)-isothiocyanate has been used:
• for fluorescence labeling of bacteria
• as a standard for calibration of fluorometer
• for the labeling of exosomes

For fluorescent labeling of proteins (e.g. immunofluorescence); H Protein labeling for gel chromatography

Packaging

100 mg in glass bottle

Safety & Documentation

Safety Information

Symbol 
GHS08  GHS08
Signal word 
Danger
Hazard statements 
Precautionary statements 
RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Protocols & Articles
Peer-Reviewed Papers
15

References

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