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F4274 Sigma-Aldrich

Fluorescein isothiocyanate isomer I

≥97.5% (HPLC)

Synonym: FITC, Fluorescein 5-isothiocyanate

  • CAS Number 3326-32-7

  • Empirical Formula (Hill Notation) C21H11NO5S

  • Molecular Weight 389.38

  •  Beilstein/REAXYS Number 1407295

  •  EC Number 222-042-0

  •  MDL number MFCD00005063

  •  PubChem Substance ID 24894860

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Properties

Related Categories Biochemicals and Reagents, Cell Biology, E-F, Fluorescein and Derivatives, Fluorescence,
InChI Key   MHMNJMPURVTYEJ-UHFFFAOYSA-N
assay   ≥97.5% (HPLC)
form   powder
mp   >360 °C (lit.)
solubility   acetone: soluble 10 mg/mL
fluorescence   λex 492 nm; λem 518 nm (green)
storage temp.   2-8°C

Description

General description

Fluorescein isothiocyanate (FITC) is widely used to attach a fluorescent label to proteins via the amine group. The isothiocyanate group reacts with amino terminal and primary amines in proteins. It has been used for the labeling of proteins including antibodies and lectins.

Application

Fluorescein isothiocyanate isomer I has been used for the staining of conidia.

Reagent for the FITC labeling of proteins; Microsequencing of proteins and peptides (HPLC)

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

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Certificate of Origin

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Milli-Q® Water Purification Solutions
Protocols & Articles
Peer-Reviewed Papers
15

References

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