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F4799 Millipore

3X FLAG® Peptide

lyophilized powder

Synonym: Met-Asp-Tyr-Lys-Asp-His-Asp-Gly-Asp-Tyr-Lys-Asp-His-Asp-Ile-Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys, ddddk peptide, dykddddk peptide

  • Empirical Formula (Hill Notation) C120H169N31O49S

  • Molecular Weight 2861.87

  •  MDL number MFCD01863911

  •  NACRES NA.32

Purchase

Properties

Related Categories FLAG Affinity Purification, FLAG System, FLAG and 3xFLAG Peptides, Molecular Biology, Plant Biotechnology,
Quality Level   200
form   lyophilized powder
concentration   (Recommended working concentration is 100 μg/ml for elute 3X FLAG fusion proteins from the ANTI-FLAG® M2 affinity gel.)
shipped in   wet ice
storage temp.   2-8°C

Description

General description

The 3X FLAG Peptide is a synthetic peptide of 23 amino acid residue. The Asp-Tyr-Lys-Xaa-Xaa-Asp motif is repeated three times in the peptide. Eight amino acids at the C-terminus make up the classic FLAG sequence (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys).

Application

For use in competitive elution of 3X FLAG® fusion proteins from the ANTI-FLAG® M2 monoclonal antibody (F1804) in solution or bound to agarose on the ANTI-FLAG® M2 agarose affinity gel (A2220). This is achieved by Affinity Chromatography.

Browse additional application references in our FLAG® Literature portal.

Preparation Note

To prepare a stock solution, dissolve in TBS (50 mMTris-HCl, pH 7.4, with 150 mM NaCl) at a concentration of 5 mg/ml.

Legal Information

ANTI-FLAG is a registered trademark of Sigma-Aldrich Co. LLC

FLAG is a registered trademark of Sigma-Aldrich Co. LLC

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Protocols & Articles

Articles

Anti-FLAG® M2 Magnetic Beads

Anti-FLAG® M2 magnetic beads provide an easy, fast and convenient method for the detection and capture of fusion proteins with the FLAG® peptide sequence. Beads are composed of a murine derived, anti...

Protocols

Immunoprecipitation of FLAG fusion proteins using monoclonal antibody affinity gels

Immunoprecipitation (IP) can be used for efficient, high-yield isolation and purification of proteins fused to the FLAG® peptide tag. IP is performed with the ANTI-FLAG® M2 affinity gel, which is a h...
Keywords: Cell disruption, Centrifugation, Electrophoresis, Gene expression, Immobilization, Immunoprecipitation, Individual protein Immunoprecipitation, Nucleic acid denaturation, PAGE, Precipitation, Purification, Sample preparations, Size-exclusion chromatography, Tagged proteins

Peer-Reviewed Papers
15

References

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