F4799 Millipore

3X FLAG® Peptide

lyophilized powder

Synonym: Met-Asp-Tyr-Lys-Asp-His-Asp-Gly-Asp-Tyr-Lys-Asp-His-Asp-Ile-Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys, ddddk peptide, dykddddk peptide

  • Empirical Formula (Hill Notation) C120H169N31O49S

  • Molecular Weight 2861.87

  •  MDL number MFCD01863911

  •  NACRES NA.32



Related Categories FLAG Affinity Purification, FLAG System, FLAG and 3xFLAG Peptides, Molecular Biology, Plant Biotechnology,
Quality Level   200
form   lyophilized powder
concentration   (Recommended working concentration is 100 μg/ml for elute 3X FLAG fusion proteins from the ANTI-FLAG® M2 affinity gel.)
shipped in   wet ice
storage temp.   2-8°C


General description

The 3X FLAG Peptide is a synthetic peptide of 23 amino acid residue. The Asp-Tyr-Lys-Xaa-Xaa-Asp motif is repeated three times in the peptide. Eight amino acids at the C-terminus make up the classic FLAG sequence (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys).


For use in competitive elution of 3X FLAG® fusion proteins from the ANTI-FLAG® M2 monoclonal antibody (F1804) in solution or bound to agarose on the ANTI-FLAG® M2 agarose affinity gel (A2220). This is achieved by Affinity Chromatography.

Browse additional application references in our FLAG® Literature portal.

Preparation Note

To prepare a stock solution, dissolve in TBS (50 mMTris-HCl, pH 7.4, with 150 mM NaCl) at a concentration of 5 mg/ml.

Legal Information

ANTI-FLAG is a registered trademark of Sigma-Aldrich Co. LLC

FLAG is a registered trademark of Sigma-Aldrich Co. LLC

Safety & Documentation

Safety Information

NONH for all modes of transport
WGK Germany 


Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Protocols & Articles


Anti-FLAG® M2 Magnetic Beads

Anti-FLAG® M2 magnetic beads provide an easy, fast and convenient method for the detection and capture of fusion proteins with the FLAG® peptide sequence. Beads are composed of a murine derived, anti...


Immunoprecipitation of FLAG fusion proteins using monoclonal antibody affinity gels

Immunoprecipitation (IP) can be used for efficient, high-yield isolation and purification of proteins fused to the FLAG® peptide tag. IP is performed with the ANTI-FLAG® M2 affinity gel, which is a h...
Keywords: Cell disruption, Centrifugation, Electrophoresis, Gene expression, Immobilization, Immunoprecipitation, Individual protein Immunoprecipitation, Nucleic acid denaturation, PAGE, Precipitation, Purification, Sample preparations, Size-exclusion chromatography, Tagged proteins

Peer-Reviewed Papers


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