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F7252 Sigma-Aldrich

Fast Green FCF

Dye content ≥85 %

Synonym: Food green 3

  • CAS Number 2353-45-9

  • Linear Formula C37H34N2O10S3Na2

  • Molecular Weight 808.85

  •  Colour Index Number 42053

  •  Beilstein/REAXYS Number 5718212

  •  EC Number 219-091-5

  •  MDL number MFCD00013053

  •  PubChem Substance ID 57654190

  •  NACRES NA.83

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Properties

Related Categories Buffers, Reagents, & Stains, Cell Biology, E-F, Hematology and Histology, Molecular Biology,
InChI Key   RZSYLLSAWYUBPE-UHFFFAOYSA-L
form   powder
composition   Dye content, ≥85%
mp   290 °C (dec.) (lit.)
solubility   H2O: 1 mg/mL, clear

Description

General description

Fast Green FCF is one of the triarylmethane dyes, that is biocompatible. This triphenylmethane color additive is mainly used in coloring food, drugs and cosmetics.

Application

Fast Green FCF has been used for in staining of menisci from rabbit and of osteochondral tissues.

Fast Green FCF dye is used for protein staining in IEF, native PAGE and SDS-PAGE. Fast Green staining is linear over a wider range of protein concentrations than Brilliant Blue R. After electrophoresis, fixing the proteins in the gel is recommended for maximum sensitivity. The gel can then be stained for 2 hr with 0.1% Fast Green FCF in either 30% (v/v) ethanol, 10% (v/v) acetic acid or in 7% acetic acid. The gel can be destained with either 30% (v/v) ethanol, 10% (v/v) acetic acid, or 7% acetic acid. The stained gel can be scanned at 625 nm. The detection sensitivity is approx. 30% that with Brilliant Blue R.

Packaging

5, 25, 100 g in glass bottle

Biochem/physiol Actions

Fast Green FCF can be considered as a safe stain to detect tumor. In recent years, this has gained importance as an important drug to treat amyloid-deposit diseases.

Safety & Documentation

Safety Information

Symbol 
GHS08  GHS08
Signal word 
Warning
Hazard statements 
Precautionary statements 
Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
2
RTECS 
BQ4425000

Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Protocols & Articles

Articles

Electrophoresis - Protein Staining Reagents

To meet the great diversity of protein analysis needs, we offer a wide selection of protein visualization (staining) reagents. EZBlue™ and ProteoSilver™, designed specifically for proteomics, also pe...
Keywords: PAGE, Proteomics

HPLC Analysis of Synthetic Food Dyes on Ascentis® Express C18

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Synthetic Food Dyes on Ascentis® Express C18
Keywords: Chromatography, High performance liquid chromatography

Peer-Reviewed Papers
15

References

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