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F9015 Sigma-Aldrich

Fluorescamine

≥98% (TLC), powder, used for detection of primary amines

Synonym: 4-Phenylspiro-[furan-2(3H),1-phthalan]-3,3′-dione

  • CAS Number 38183-12-9

  • Empirical Formula (Hill Notation) C17H10O4

  • Molecular Weight 278.26

  •  Beilstein/REAXYS Number 921143

  •  EC Number 253-814-5

  •  MDL number MFCD00005928

  •  PubChem Substance ID 24894984

  •  NACRES NA.32

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Properties

Related Categories Biochemicals and Reagents, Chemical Biology, Chemical Synthesis, Derivatization agents, Fluorescent Labels,
assay   ≥98% (TLC)
form   powder
color   off-white to yellow
mp   153-157 °C (lit.)
solubility   acetone: 50 mg/mL
storage temp.   room temp
SMILES string   O=C1OC2(OC=C(C2=O)c3ccccc3)c4ccccc14
InChI   1S/C17H10O4/c18-15-13(11-6-2-1-3-7-11)10-20-17(15)14-9-5-4-8-12(14)16(19)21-17/h1-10H
InChI key   ZFKJVJIDPQDDFY-UHFFFAOYSA-N

Description

Packaging

1 g in glass bottle

100, 250 mg in glass bottle

Application

Non-fluorescent reagent that reacts readily under mild conditions with primary amines in amino acids and peptides to form stable, highly fluorescent compounds. Low background due to hydrolysis. Useful for the fluorometric assay of amino acids, protein, and proteolytic enzymes. Effectively blocks newly generated amino termini in protein sequence analyses.

Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 3
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles

Articles

Electrophoresis - Protein Staining Reagents

To meet the great diversity of protein analysis needs, we offer a wide selection of protein visualization (staining) reagents. EZBlue™ and ProteoSilver™, designed specifically for proteomics, also pe...
Keywords: PAGE, Proteomics

Peer-Reviewed Papers
15

References

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