F9037 Sigma-Aldrich


≥99.5% (GC), BioReagent, for molecular biology

Synonym: Amide C1, Formic amide



Related Categories Acids and Bases, Biochemicals and Reagents, Core Bioreagents, Denaturation, Life Science Reagents for DNA/RNA Electrophoresis,
grade   for molecular biology
vapor density   1.55 (vs air)
vapor pressure   0.08 mmHg ( 20 °C)
  30 mmHg ( 129 °C)
product line   BioReagent
assay   ≥99.5% (GC)
form   liquid
autoignition temp.   932 °F
refractive index   n20/D 1.447 (lit.)
bp   210 °C(lit.)
mp   2-3 °C (lit.)
density   1.134 g/mL at 25 °C (lit.)
suitability   suitable for nucleic acid hybridization
storage temp.   2-8°C


General description

Formamide is a clear liquid amide derived from formic acid. It can be used as a cryoprotectant and gel-stabilizer. It is known to destabilize nucleic acid duplexes, making it useful for preparing hybridization buffers for RNA and DNA applications.


Formamide is suitable for use in hybridization buffers made using standard recipes. It was used in preparation of brain sections of mice for BrdU immunohistochemistry and FISH analysis of HeLa cells. It was used to prepare the polyacrylamide gel for analysis of PCR products using denaturing gradient gel electrophoresis.

Formamide destabilizes nucleic acid duplexes and may be used, typically, at a concentration of 50%, in hybridization protocols requiring lower hybridization temperatures.

Other Notes

The product has been deionized for molecular biology use and packaged under argon gas in amber glass bottles to protect it from oxidation.

Safety & Documentation

Safety Information

GHS08  GHS08
Signal word 
Hazard statements 
Precautionary statements 
NONH for all modes of transport
WGK Germany 
Flash Point(F) 
347 °F
Flash Point(C) 
175 °C


Certificate of Analysis


Certificate of Origin

Protocols & Articles


Formamide Molecular Biology Reagent

Formamide is a reagent that is an ionizing solvent in aqueous buffers. It is widely utilized in biochemistry and molecular biology, particularly in nucleic acids research. Procedures have been report...
Keywords: Biochemistry, Buffers, Capillary electrophoresis, Catalysis, Degradations, Dehydration reaction, Electrophoresis, Mass spectrometry, Melting, Molecular biology, Northern blot, Nucleic acid denaturation, Nucleic acid hybridization, Oxidations, Phase transitions, Polymerase chain reaction, Sequencing

GC Analysis of Class 2 Residual Solvents on OVI-G43

From our library of Articles, Sigma-Aldrich presents GC Analysis of Class 2 Residual Solvents on OVI-G43
Keywords: Chromatography, Flame ionization detector, Gas chromatography, Pharmaceutical, Size-exclusion chromatography

MSMLS Online Plate Map

Metabolite descriptors included with the software download upon purchase of the product include: Name, Parent CID, molecular formula, molecular weight, CAS, ChEBI, HMDB ID, PubChem Compound and Subst...
Keywords: Mass spectrometry, Metabolites

Peer-Reviewed Papers


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