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G1393 Sigma-Aldrich

Gelatin solution

Type B, 2% in H2O, tissue culture grade, BioReagent, suitable for cell culture

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Properties

Related Categories Attachment Factors, Attachments Factors, Cell Culture, Core Bioreagents, Life Science Reagents for Cell Culture,
biological source   bovine hide
sterility   sterile
type   Type B
product line   BioReagent
form   solution
packaging   pkg of 100 mL
  pkg of 20 mL
concentration   2% in H2O, tissue culture grade
surface coverage   100‑200 μg/cm2
impurities   endotoxin, tested
suitability   suitable for cell culture
storage temp.   2-8°C

Description

Components

Gelatin is a heterogeneous mixture of water-soluble proteins of high average molecular masses, present in collagen. Proteins are extracted by boiling the relevant skin, tendons, ligaments, bones, etc. in water. Type A gelatin is derived from acid-cured tissue. Type B is derived from lime-cured tissue.

Application

This product is recommended for use as a cell culture substratum at 1-5 μg/cm2 or 0.5-50 μg/mL. The optimal concentration does depend on cell type as well as the application and research objectives.

Gelatin has been used in many applications. It has use in coating cell culture to improve attachment of cells, being added to PCR to stabilize Taq DNA, as a blocking reagent in Western blotting, ELISA, and immunochemistry, and as a component of media for species differentiation in bacteriology. As a biocompatible polymer, it has used as a delivery vehicle for release of active biomolecules and in generation of scaffolds for tissue engineering applications. It has also been used to study long-chain fatty acid-induced changes in gene expression in neonatal cardiac monocytes and to study to test mobilization of capillary endothelium in vitro induced by effectors of angiogenesis in vivo.

Caution

Dry gelatin, when stored in airtight containers at room temperature, will remain unchanged for many years. When heated at 100°C in the presence of air, it swells becomes soft and disintegrates to a carbonaceous mass with evolution of pyridine bases and ammonia.

Preparation Note

This product is derived lime-cured tissue bovine skin, and is a 2% solution in water.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
2

Documents

Certificate of Analysis

Certificate of Origin


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Cell Culture Guide
Protocols & Articles

Articles

3d Cell Culture: Tools and Techniques

Cell culture techniques are ubiquitous in areas of developmental biology, drug discovery, regenerative medicine and protein production. Since the introduction of cell culture techniques, cells have b...
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Attachment Factors for 3-Dimensional Cell Culture

Three-dimensional (3D) extracellular matrix-based cell culture systems are being used as research tools for understanding normal and disease systems, and for drug screening in vitro. The extracellula...
Efrat Manaster
BioFiles 2008, 3.8, 16.
Keywords: Adhesion, Angiogenesis, Biomaterials, Cancer, Cell biology, Cell culture, Clinical, Gene expression, Genetic, Genetics, Genomics, Growth factors, Hormones, Molecular biology, Morphogenesis

Cancer Stem Cells: New Targets for Cancer Therapy

Interested in receiving more content like this? Join over 8,000 of your peers who have already signed up for the Cell Informer newsletter.
Keywords: Cancer, Cell attachment, Cell culture, Cell proliferation, Gastrointestinal, Gene expression, Transcription, transformation

Gelatin Cell Attachment Protocol

From our library of Articles, Sigma-Aldrich presents Gelatin Cell Attachment Protocol
George Sitterley
BioFiles 2008, 3.8, 10.
Keywords: Sterilizations

Gelatin

Gelatin is a heterogeneous mixture of water-soluble proteins of high average molecular weights, present in collagen. The proteins are extracted by boiling skin, tendons, ligaments, bones, etc. in wat...
George Sitterley
BioFiles 2008, 3.8, 10.
Keywords: Biomaterials, Cell attachment, Cell culture, Culture media, Enzyme-linked immunosorbent assay, Hemostatics, Immunohistochemistry, Pharmaceutical, Polymerase chain reaction, Veterinary, Western blot

What is the Extracellular Matrix?

The extracellular matrix (ECM) is secreted by cells and surrounds them in tissues. It has long been understood to be the structural support for cells since its characteristics set the characteristics...
Keywords: Adhesion, Cell culture, Cellular processes, Epimerizations, Growth factors, Immobilization, Sulfations

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Cell culture has become one of the most fundamental techniques for modeling biological systems, and is of increasing importance in the biotechnology and pharmaceutical sectors as well as an essential...
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Peer-Reviewed Papers
15

References

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