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G7153 Sigma-Aldrich

Guanidine hydrochloride

BioXtra, ≥99.5% (titration)

Synonym: Aminoformamidine hydrochloride, Aminomethanamidine hydrochloride, Guanidinium chloride

  • CAS Number 50-01-1

  • Linear Formula NH2C(=NH)NH2 · HCl

  • Molecular Weight 95.53

  •  Beilstein/REAXYS Number 3591990

  •  EC Number 200-002-3

  •  MDL number MFCD00013026

  •  PubChem Substance ID 24895295

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Properties

Related Categories Biochemicals and Reagents, Cell Lysis and Protein Extraction Reagents, Chaotropic Agents, Core Bioreagents, Denaturation,
InChI Key   PJJJBBJSCAKJQF-UHFFFAOYSA-N
Quality Level   ELITE
product line   BioXtra
assay   ≥99.5% (titration)
impurities   Insoluble matter, passes filter test
ign. residue   ≤0.05%
loss   ≤0.05% loss on drying, HV, 20°C
pH   4.5-6.0 (6 M in H2O)
mp   180-185 °C (lit.)
solubility   H2O: 6 M, clear, colorless
anion traces   sulfate (SO42-): ≤0.005%
cation traces   Al: ≤0.0005%
  As: ≤0.0001%
  Ba: ≤0.0005%
  Bi: ≤0.0005%
  Ca: ≤0.001%
  Cd: ≤0.0005%
  Co: ≤0.0005%
  Cr: ≤0.0005%
  Cu: ≤0.0005%
  Fe: ≤0.0005%
  K: ≤0.005%
  Li: ≤0.0005%
  Mg: ≤0.0005%
  Mn: ≤0.0005%
  Mo: ≤0.0005%
  Na: ≤0.005%
  Ni: ≤0.0005%
  Pb: ≤0.0005%
  Sr: ≤0.0005%
  Zn: ≤0.0005%

Description

Application

Strong chaotropic agent useful for the denaturation and subsequent refolding of proteins. This strong denaturant can solubilize insoluble or denatured proteins such as inclusion bodies. This can be used as the first step in refolding proteins or enzymes into their active form. Urea and dithiothreitol (DTT) may also be necessary.

Safety & Documentation

Safety Information

Symbol 
GHS07  GHS07
Signal word 
Warning
Hazard statements 
RIDADR 
NONH for all modes of transport
WGK Germany 
1
RTECS 
MF4300000

Documents

Certificate of Analysis

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Certificate of Origin

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Protocols & Articles
Peer-Reviewed Papers
15

References

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