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G8540 Sigma

L-Glutamine

meets USP testing specifications, cell culture tested, 99.0-101.0%, from non-animal source

Synonym: (S)-2,5-Diamino-5-oxopentanoic acid, L-Glutamic acid 5-amide, Levoglutamide

  • CAS Number 56-85-9

  • Linear Formula H2NCOCH2CH2CH(NH2)CO2H

  • Molecular Weight 146.14

  •  Beilstein Registry Number 1723797

  •  EC Number 200-292-1

  •  MDL number MFCD00008044

  •  eCl@ss 32160406

  •  PubChem Substance ID 24895342

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Properties

Related Categories Amino Acids, Amino Acids & Vitamins, Amino Acids and Vitamins, Amino acids, Bioactives/Supplements,
biological source   non-animal source
InChI Key   ZDXPYRJPNDTMRX-VKHMYHEASA-N
assay   99.0-101.0%
form   powder
impurities   endotoxin, tested
mp   185 °C (dec.)(lit.)
solubility   H2O: 25 mg/mL
suitability   cell culture tested
  meets USP testing specifications
Featured Industry   Agriculture
Gene Information   rat ... Ggt1(116568)

Description

Biochem/physiol Actions

L-Glutamine is an essential amino acid that is a crucial component of culture media that serves as a major energy source for cells in culture. L-Glutamine is very stable as a dry powder and as a frozen solution. In liquid media or stock solutions, however, L-glutamine degrades relatively rapidly. Optimal cell performance usually requires supplementation of the media with L-glutamine prior to use.

Glutamine provides the necessary nitrogen, carbon and the reducing equivalents required for cell growth and division during cell culture. L-Glutamic acid aids the incorporation of NH4+ into biomolecules.

Packaging

1 kg in poly bottle

10 mg in glass bottle

25, 100 g in poly bottle

Application

L-Glutamine has been used as one of the components for the preparation of calcium-free mCZB medium. It has also been used as a supplement in a culture medium for rat hippocampal slices.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
1
RTECS 
MA2275100

Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
How do I find price and availability?
There are several ways to find pricing and availability for our products.Once you log onto our website, you will find the price and availability displayed on the product detail page.You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 orview local office numbers.
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
Is the molecular weight of L-Glutamine 146.14 or 146.15? The label information changed.
Based on a molecular formula of C5H10N2O3, the molecular weight to seven significant figures is 146.1456. Over the years, this value may have been reported in Sigma-Aldrich documentation or on labels as either 146.15 (by rounding up) or 146.14 (by truncation). The material itself has not changed.
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Cell Culture Guide
Protocols & Articles

Articles

HPLC Analysis of Glutamine Enantiomers on Astec® CHIROBIOTIC® T (23 °C)

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Glutamine Enantiomers on Astec® CHIROBIOTIC® T (23 °C)
Keywords: Chromatography, High performance liquid chromatography

MSMLS Online Plate Map

Metabolite descriptors included with the software download upon purchase of the product include: Name, Parent CID, molecular formula, molecular weight, CAS, ChEBI, HMDB ID, PubChem Compound and Subst...
Keywords: Mass spectrometry, Metabolites

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L-Glutamine in Cell Culture

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Peer-Reviewed Papers
15

References

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G7513 L-Glutamine solution, 200 mM, solution, sterile-filtered, BioXtra, suitable for cell culture

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