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G8773 Sigma-Aldrich

Glycerol

for electrophoresis, ≥99%

Synonym: 1,2,3-Propanetriol, Glycerin

  • CAS Number 56-81-5

  • Linear Formula HOCH2CH(OH)CH2OH

  • Molecular Weight 92.09

  •  Beilstein/REAXYS Number 635685

  •  EC Number 200-289-5

  •  MDL number MFCD00004722

  •  PubChem Substance ID 24895352

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Properties

grade   for electrophoresis
vapor density   3.1 (vs air)
vapor pressure   <1 mmHg ( 20 °C)
assay   ≥99%
autoignition temp.   698 °F
impurities   ≤0.005% Chlorinated compounds (as Cl)
refractive index   n20/D 1.474 (lit.)
bp   182 °C/20 mmHg (lit.)
mp   20 °C (lit.)
density   1.25 g/mL (lit.)
anion traces   sulfate (SO42-): ≤0.005%
cation traces   heavy metals (as Pb): ≤5 ppm
SMILES string   OCC(O)CO
InChI   1S/C3H8O3/c4-1-3(6)2-5/h3-6H,1-2H2
InChI key   PEDCQBHIVMGVHV-UHFFFAOYSA-N

Description

Application

Glycerol is used both in sample preparation and gel formation for polyacrylamide gel electrophoresis. Glycerol (5-10%) increases the density of a sample so that the sample will layer at the bottom of a gel’s sample well. Glycerol is also used to aid in casting gradient gels and as a protein stabilizer and storage buffer component.

Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
1
RTECS 
MA8050000
Flash Point(F) 
320 °F
Flash Point(C) 
160 °C
Protocols & Articles
Peer-Reviewed Papers
15

References

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