• USA Home
  • G9277 - Guanidine thiocyanate

G9277 Sigma-Aldrich

Guanidine thiocyanate

for molecular biology, ≥99%

Synonym: Guanidinium rhodanide, Guanidinium thiocyanate

  • CAS Number 593-84-0

  • Linear Formula NH2C(=NH)NH2 · HSCN

  • Molecular Weight 118.16

  •  Beilstein/REAXYS Number 3563461

  •  EC Number 209-812-1

  •  MDL number MFCD00013027

  •  PubChem Substance ID 24895367

  •  NACRES NA.31



Related Categories Biochemicals, Biochemicals and Reagents, Chaotropic Agents, Core Bioreagents, DNA & RNA Purification,
grade   for molecular biology
assay   ≥99%
mp   120-122 °C (lit.)
cation traces   Ba: ≤5 ppm
  Fe: ≤5 ppm
  K: ≤0.005%
  Na: ≤0.5%
  heavy metals (as Pb): ≤5 ppm
Featured Industry   Agriculture
foreign activity   DNase, RNase, none detected
SMILES string   SC#N.NC(N)=N
InChI   1S/CH5N3.CHNS/c2-1(3)4;2-1-3/h(H5,2,3,4);3H



Guanidine thiocyanate has been used:
• for the lysis of colorectal adenocarcinoma cell line prior to RNA isolation
• as a PCR inhibitor in triplex mitochondrial DNA (mtDNA) qPCR assay
• for DNA extraction from cerebrospinal fluid (CSF)

Chaotropic agent and strong denaturant; solubilizes cells.


100, 250, 500 g in poly bottle

Biochem/physiol Actions

Guanidine thiocyanate is a denaturing agent and is used routinely in RNA isolation. It is used as a storage buffer for whole blood samples. Guanidine thiocyanate inactivates nucleases and is ideal for storing and freezing fecal samples for DNA studies. It is used in combination with phenol−chloroform in RNA extraction.

Safety & Documentation

Safety Information

Signal word 
Hazard statements 
Supplemental Hazard Statements 
Contact with acids liberates very toxic gas.
Personal Protective Equipment 
UN 1759 8 / PGIII
WGK Germany 


Certificate of Analysis (COA)

Please Enter a Lot Number

Certificate of Origin (COO)

Please Enter a Lot Number

pHast Pack
Protocols & Articles

Related Content

Agriculture | Crop Protection Workflow

Pesticides are an essential component in modern agriculture and enable growers to fully realize the yield potential of their seeds. From compound discovery to active ingredient development, our chemi...
Keywords: Agriculture, Building blocks, Gas chromatography, Gas chromatography mass spectrometry, High performance liquid chromatography, Liquid chromatography mass spectrometry, Mass spectrometry, Organic synthesis, Pesticides, Polymerase chain reaction - quantitative, Purification, Solvents

Agriculture | Genotyping

Sigma-Aldrich’s chemistry expertise can help customers develop and optimize their leaf and seed genotyping workflow to reduce cost per data point while increasing throughput rates. In addition, our g...
Keywords: Amplification, Polymerase chain reaction, Polymerase chain reaction - quantitative, Sequencing, Whole genome amplification

Plant Breeding Workflow - Collaborating to Empower Yield Improvements

Sigma-Aldrich products are aligned to the discovery, development and production phases of the plant breeding workflow, allowing you to develop and produce new crop varieties faster. With materials ma...
Keywords: Building blocks, Mass spectrometry, Organic synthesis, Polymerase chain reaction, Polymerase chain reaction - quantitative, Vitamins

Peer-Reviewed Papers


Related Products

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?