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GF197 Sigma-Aldrich

Vaccinia Virus B18R protein, recombinant expressed in HEK 293 cells, Carrier-Free

recombinant, expressed in HEK 293 cells, endotoxin tested, cell culture tested, carrier-free

Synonym: B18, Soluble interferon alpha/beta receptor B18

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Properties

Related Categories Cell Biology, Cell Signaling and Neuroscience, Cytokines and Growth Factors, Cytokines, Growth Factors and Hormones
recombinant   expressed in HEK 293 cells
assay   ≥98% (SDS-PAGE)
form   liquid
potency   Supports transfection of Simplicon-based TagGFP RNA
mol wt   mol wt 50 kDa (glycosylated)
suitability   cell culture tested
  endotoxin tested
shipped in   dry ice
storage temp.   −70°C

Description

Application

B18R protein can be used with the Simplicon RNA Reprogramming Technology (Catalog No. SCR550) to create “foot-print” free human iPS cells at the highest efficiency without unwanted RNA degradation caused by cellular IFN responses.

Biochem/physiol Actions

B18R is a vaccinia virus gene product that acts as a decoy receptor for Type I Interferons (IFNs). B18R binds type I IFNs more avidly than their receptor. This protein has been shown to inhibit all IFN-α subtypes and IFN-β, but not type II or III IFNs. B18R enables increased cell viability during RNA transfection protocols designed to convert human somatic donor cells into iPSCs via direct delivery of synthetic mRNA′s. As a type I IFN receptor, B18R has a broad species specificity. It has significant regions of homology with the alpha subunits of the mouse, human, and bovine type I IFN receptors. Binding of soluble recombinant B18R to cells protected the cultures from IFN and allowed vaccinia virus replication. B18R can be used with the Simplicon RNA Reprogramming Technology (Cat. No. SCR550) to create “foot-print” free human iPS cells at the highest efficiency without unwanted RNA degradation caused by cellular IFN responses.

Physical form

This product is supplied as a 50 μg liquid solution, with 10 mM phosphate, 0.5 M NaCl, pH 7.5 buffer. It is aseptically filled. The protein concentration is 300-700 μg/ml (by the BCA method). For exact concentration see the product’s Certificate of Analysis.

General description

Recombinant B18R from vaccinia virus is expressed in human HEK 293 cells as an N-terminally his-tagged glycoprotein with a calculated molecular mass of 38.4 kDa. The DTT-reduced protein migrates as a ~50 kDa polypeptide on SDS-PAGE due to glycosylation. The non-reduced protein migrates as a ~46 kDa polypeptide on SDS-PAGE due to internal cystines. This protein is manufactured in human cells, with no serum. The human cells expression system allows human-like glycosylation and folding, and often supports better stability of the protein in culture.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Cell Culture Guide
Protocols & Articles

Articles

Simplicon™ In Vivo Protein Expression System

Simplicon™ is a novel system that generates immediate, high-sustained protein expression of multiple genes into transfected human cells without the risk of genome integration. The technology employs ...
Keywords: Cloning, Culture media, Gene expression, Genetic, Microscopy, Transfection

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