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H0887 Sigma-Aldrich

HEPES solution

1 M, pH 7.0-7.6, sterile-filtered, BioReagent, suitable for cell culture

Synonym: N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid)

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Properties

Related Categories Biochemicals and Reagents, Biological Buffers, Buffer Convenience Packaging, Buffer Solutions, Buffers A to Z,
InChI Key   JKMHFZQWWAIEOD-UHFFFAOYSA-N
Quality Level   PREMIUM
sterility   sterile-filtered
product line   BioReagent
form   liquid
concentration   1 M
impurities   endotoxin, tested
pH   7.0-7.6
useful pH range   6.8 - 8.2
pH range   7.0 - 7.6
suitability   suitable for cell culture
storage temp.   2-8°C

Description

General description

HEPES has been described as one of the best all-purpose buffers available for biological research. At biological pH, the molecule is zwitterionic, and is effective as a buffer at pH 6.8 to 8.2 (pKa 7.55). It is typically used in cell culture at concentration between 5mM to 30 mM. HEPES has been used in a wide variety of applications, including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg.

Application

HEPES has been used as a component of:
• Hank’s balanced salt solution, Dulbecco’s modified eagle’s medium and no-glucose DMEM, which are used for the preparation of tissue slices
• Buffers used in localized conductance measurements on the basolateral side of culture inserts to examine cation selectivity

Caution

Concentrations of HEPES over 40mM may be toxic to some cell types.

Preparation Note

Prepared from HEPES free acid and cell culture grade water.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
1

Documents

Certificate of Analysis

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Cell Culture Guide

Milli-Q® Water Purification Solutions
Protocols & Articles

Articles

Attachment-Dependent Cell Growth and Cell Proliferation in the MultiScreen Assay System

Numerous bioassays utilize cell cultures to quantitate the effects of potential growth modifiers. These assays are typically performed in 96-well microwell plates for “convenience” and are usually fo...
Keywords: Adhesion, Cell attachment, Cell culture, Cell proliferation, Culture media, Filtration, Growth factors, Scintillation, Transduction

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Cell Culture Troubleshooting Guide

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Peer-Reviewed Papers
15

References

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H4034 HEPES, BioPerformance Certified, ≥99.5% (titration), cell culture tested
H3784 HEPES sodium salt, BioPerformance Certified, suitable for cell culture, abs.
08168 Timestrip Plus 8 °C

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