H9039 Sigma-Aldrich


from bovine, ≥90%

Synonym: Chloro(protoporphyrinato)iron(III), Chlorohemin, Chloroprotoporphyrin IX iron(III), Ferriprotoporphyrin IX chloride, Hemin(chloride)

  • CAS Number 16009-13-5

  • Empirical Formula (Hill Notation) C34H32ClFeN4O4

  • Molecular Weight 651.94

  •  Beilstein/REAXYS Number 5229914

  •  EC Number 240-140-1

  •  MDL number MFCD00010726

  •  PubChem Substance ID 329815257

  •  NACRES NA.61



Related Categories Application Index, Biochemicals and Reagents, Cell Biology, Enzymes, Inhibitors, and Substrates, Hemin,
Quality Level   200
biological source   bovine
assay   ≥90%
form   powder
application(s)   toxicology assay: suitable
solubility   1.4 M NaOH: soluble 25 mg/mL
storage temp.   2-8°C
SMILES string   Cc1c(CCC(O)=O)c2cc3c(CCC(O)=O)c(C)c4cc5nc(cc6c(C)c(C=C)c(cc1n2)n6[Fe](Cl)n34)c(C=C)c5C
InChI   1S/C34H34N4O4.ClH.Fe/c1-7-21-17(3)25-13-26-19(5)23(9-11-33(39)40)31(37-26)16-32-24(10-12-34(41)42)20(6)28(38-32)15-30-22(8-2)18(4)27(36-30)14-29(21)35-25;;/h7-8,13-16H,1-2,9-12H2,3-6H3,(H4,35,36,37,38,39,40,41,42);1H;/q;;+3/p-3/b25-13-,26-13-,27-14-,28-15-,29-14-,30-15-,31-16-,32-16-;;


General description

Heme is a small molecule present either free or bound to hemoglobin in the bloodstream of mammals. This is an alternative source of iron within the host that contains a porphyrin ring containing a Fe2+ ion called hemin.


Hemin was used to study conjugation, characterization and toxicity of lipophosphoglycan-polyacrylic acid conjugate for vaccination against leishmaniasis.


1, 10, 100 g in glass bottle

Safety & Documentation

Safety Information

Personal Protective Equipment 
NONH for all modes of transport
WGK Germany 
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable


Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
What is the solubility of Hemin, Product H9039?
Hemin can be dissolved in basic solution.A 25 mg/ml solution can be prepared in 1.4 M ammonium hydroxide with heating to 95°C for less than 5 minutes. Hemin is also expected to be soluble in a sodium hydroxide solution. Hemin has been reported to be soluble at 1 mM concentration in DMSO, per Tomlinson and Ferguson, Proceedings of the National Academy of Sciences, 97, 5156-5160 (2000).A 0.25% hemin solution can also be prepared in 50% triethanolamine solution.
How stable isHemin, Product H9039, in solution?
Sigma-Aldrich has not evaluated the solution stability of hemin solutions.Also, we have not found any information on solution storage in the literature. We recommend that solutions be prepared fresh.
What is the shelf life for Hemin, Product H9039?
There is no shelf life date assigned to this product. The guarantee of quality is for one year from the time of shipment for bottles stored as recommended.
What are some applications for Hemin, Product H9039?
Hemin-agarose has reportedly been prepared for use in affinity chromatography of heme-binding proteins.See Tsutsui, K., Affinity chromatography of heme-binding proteins: Synthesis of hemin-agarose. Meth. Enzymol. 123, 331, (1986). Sigma supplies a hemin agarose, product H6390. Hemin can also be used as a component in culture media for the cultivation of bacteria, such as Leishmania tarentolae.
How do I find price and availability?
There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 or view local office numbers.
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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