• USA Home
  • I3786 - Protease Inhibitor Cocktail, Animal Component Free

I3786 Sigma-Aldrich

Protease Inhibitor Cocktail, Animal Component Free

for use with mammalian cell and tissue extract, DMSO solution

  •  NACRES NA.77



General description

Components of this cocktail are of non-animal origin and no animal products were used in the component production process. All vessels and instruments used for the cocktail production are dedicated for animal component free production and have never encountered an animal product. Aprotinin used in this cocktail is a recombinant bovine protein expressed in plants (Nicotiana).

This protease inhibitor cocktail has been optimized and tested for mammalian cell and tissue extracts.


Inhibits serine, cysteine, aspartic proteases, and aminopeptidases.


Animal component free (ACF). Optimized and tested for general use. Can also be used for purification of histidine-tagged proteins, as chelating agents have been omitted.


Pepstatin A

Physical form

The components in this ACF protease inhibitor cocktail do not originate from animal sources and can thus be used in the production of animal-free proteins.

This ACF cocktail is supplied as a ready-to-use solution in DMSO. One ml of the cocktail solution is recommended for the inhibition of endogenous enzymes found in 100 mL of lysate from 20 g (wet weight) of bovine liver, or 10 mL of cell lysate obtained from CHO cells at a cell density of 108 cells per mL.

Safety & Documentation

Safety Information

NA 1993 / PGIII
WGK Germany 
Flash Point(F) 
188.6 °F - closed cup
Flash Point(C) 
87 °C - closed cup


Certificate of Analysis (COA)

Please Enter a Lot Number

Certificate of Origin (COO)

Please Enter a Lot Number
Protocols & Articles


Protease Inhibitor Cocktails References

Our portfolio of protease inhibitor cocktails are frequently cited in peer-reviewed scientific literature, suggesting their superior quality and excellent track record. This page lists a sampling of ...
Keywords: AGE, Adhesion, Aerobic, Affinity chromatography, Alzheimer Disease, Amplification, Angiogenesis, Anti-inflammatory agents, Antibiotics, Antimicrobials, Antitumor agents, Antivirals, Apoptosis, Atomic absorption spectroscopy, Autophagy, Biochemistry, Biological processes, Buffers, Cancer, Cardiovascular, Catalysis, Cell biology, Cell disruption, Cell proliferation, Cell signaling, Cellular processes, Central Nervous System, Chromatin immunoprecipitation, Chromatography, Clinical, Cloning, Cytotoxins, DNA replication, Degradations, Detergents, Diabetes, Diagnostic, Diseases, Dopamine agents, Drug discovery, Eliminations, Environmental, Enzyme-linked immunosorbent assay, Gas chromatography, Gastrointestinal, Gene expression, Genetic, Genetics, Gluconeogenesis, Glycosylations, Growth factors, Hormones, Hydration reaction, Hydroxylations, Immobilization, Immunology, Immunoprecipitation, Inflammation, Ligands, Lipid Metabolism, Lipid peroxidation, Mass spectrometry, Medicinal chemistry, Metabolism, Metabolites, Methylations, Microarray Analysis, Microbiology, Microscopy, Molecular biology, Morphogenesis, Neurology, Neuroscience, Neurotransmission, Neurotransmitters, Normal-phase chromatography, Nutrition, Obesity, Oncology, PAGE, Parkinson Disease, Peroxidations, Pharmaceutical, Phosphorylations, Photosynthesis, Physiological Processes, Polymerase chain reaction, Polymorphisms, Proteomics, Purification, Recombination, Reductions, Reproduction, Respiratory, Reversible addition-fragmentation chain transfer polymerizations, Sample preparations, Scanning electron microscopy, Schizophrenia, Separation, Sequencing, Sonication, Transcription, Transduction, Veterinary, Vitamins, Western blot, transformation

Peer-Reviewed Papers


Related Products

related product

Product #


Add to Cart

P8340 Protease Inhibitor Cocktail, for use with mammalian cell and tissue extracts, DMSO solution
I3911 Protease Inhibitor Cocktail, Animal Component Free, for general use, lyophilized powder
06693 Timestrip Plus -20 °C

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?