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K1377 Sigma-Aldrich

Kanamycin sulfate from Streptomyces kanamyceticus

powder, BioReagent, suitable for cell culture, suitable for plant cell culture

Synonym: Kanamycin A, Kanamycin sulfate salt

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Properties

Related Categories A - K, Aminoglycosides, Antibacterial, Antibiotics, Antibiotics A to Z,
biological source   Streptomyces kanamyceticus
InChI Key   OOYGSFOGFJDDHP-KMCOLRRFSA-N
product line   BioReagent
form   powder
potency   ≥750 μg per mg (dry basis)
application(s)   cell culture | plant: suitable
impurities   ≤5% kanamycin B
color   white to off-white
solubility   H2O: 10-50 mg/mL (As a stock solution. Stock solutions should be stored at 2-8°C. Stable at 37°C for 5 days.)
suitability   suitable for cell culture
  suitable for plant cell culture
Featured Industry   Agriculture
Mode of action   protein synthesis | interferes
antibiotic activity spectrum   Gram-negative bacteria
  Gram-positive bacteria
  mycobacteria
  mycoplasma

Description

General description

Chemical structure: aminoglycoside

Kanamycin sulfate is a broad spectrum aminoglycoside-antibiotic derived from Streptomyces kanamyceticus. It is used as an additive in culture media for the isolation of group D streptococci on Kanamycin Esculin Azide Agar and for selection of transformed plant cells containing the neomycin phosphotransferase on a kanamycin-medium. Kanamycin sulfate can also be used as a selection agent for cells transformed with kanamycin B resistance gene. It is recommended for use in cell culture applications at 100μg/mL.

Application

Kanamycin sulfate from Streptomyces kanamyceticus has been used in tissue culture media.

Packaging

1, 5, 25 g in poly bottle

Biochem/physiol Actions

Mode of Action: The product acts by binding to the 70S ribosomal subunit, inhibiting translocation and eliciting miscoding.

Mode of Resistance:Aminoglycoside-modifying enzymes (including acetyltransferase, phosphotransferase, nucleotidyltransferase) can alter this antibiotic, preventing its interaction with ribosomes.

Antimicrobial spectrum: Kanamycin sulfate is effective against gram-negative and gram-postiive bacteria, and mycoplasma.

Caution

Solutions are stable at 37°C for approximately 5 days. Aqueous stock solutions can be stored at 2-8°C for long term storage.

Preparation Note

Kanamycin sulfate is soluble in water at 50 mg/mL, yielding a clear solution. It is practically insoluble in alcohol, acetone, chloroform, ether and ethyl acetate. A 1% solution in water has a pH of 6.5 to 8.5. Sterile solutions can be prepared by a sterile filtration, through a .2μm filter.

Safety & Documentation

Safety Information

Symbol 
GHS08  GHS08
Signal word 
Danger
Hazard statements 
Precautionary statements 
RIDADR 
NONH for all modes of transport
WGK Germany 
2
RTECS 
NZ3225030

Documents

Certificate of Analysis

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Certificate of Origin

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Cell Culture Guide

Milli-Q® Water Purification Solutions
Protocols & Articles

Articles

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Peer-Reviewed Papers
15

References

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