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KCQS01 Sigma-Aldrich

KiCqStart® SYBR® Green qPCR ReadyMix

Low ROX, for ABI and Stratagene instruments

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Properties

Related Categories Fast QPCR, KiCqStart qPCR ReadyMix, Molecular Biology, PCR/Amplification, Quantitative PCR,
form   liquid
feature   hotstart
storage condition   protect from light
color   colorless
compatibility   for use with ABI 7500 Fast
  for use with ABI 7500
  for use with ABI QuantStudio
  for use with ABI ViiA 7
  for use with Agilent AriaMx
  for use with Douglas Scientific IntelliQube
  for use with Qiagen Rotor-Gene Q
  for use with Strategene Mx3000P
  for use with Strategene Mx3005P
  for use with Strategene Mx4000
shipped in   dry ice
storage temp.   −20°C

Description

General description

KiCqStart SYBR Green qPCR ReadyMix is a 2X concentrated, ready-to-use reaction cocktail that contains all components, except primers and template, for real-time quantitative PCR (qPCR) This unique combination of proprietary buffer, stabilizers, and Hot-Start Taq DNA polymerase delivers maximum PCR efficiency, sensitivity, specificity and robust fluorescent signal using fast, or conventional, cycling protocols with SYBR Green qPCR.

Highly specific amplification is crucial to successful qPCR with SYBR Green I dye technology because this dye binds to and detects any dsDNA generated during amplification. Hot-Start Taq DNA polymerase is antibody mediated to be inactive prior to the initial PCR denaturation step. The optimized formulation includes SYBR Green I dye, an antibody-mediated Hot-Start Taq DNA polymerase, dNPTs, MgCl2 and proprietary buffers and stabilizers.

Application

Instrument Compatibility:
Different real-time PCR systems employ different strategies for normalization of fluorescent signals and correction of well-to-well optical variations. It is critical to match the appropriate qPCR reagent to your specific instrument. KiCqStart SYBR Green qPCR ReadyMix, Low ROX is available for the following platforms:
• Agilent AriaMx
• Applied Biosystems® 7500
• Applied Biosystems® 7500 Fast
• Applied Biosystems® ViiA 7
• Applied Biosystems® Quant Studio
• Douglas Scientific IntelliQube
• QIAGEN Rotor-Gene® Q
• Stratagene Mx3000P®
• Stratagene Mx3005P®
• Stratagene Mx4000

PCR applications:
• Gene expression
• DNA quantitation
• CHiP

Features and Benefits

• Assay results in as little as 33 minutes
• Highly efficient and sensitive real-time PCR results
• Little/no optimization required

Components

2X reaction buffer containing optimized concentrations of MgCl2, dNTPs (dATP, dCTP, dGTP, dTTP), KiCqStart Taq DNA Polymerase, SYBR Green dye, ROX Reference Dye (for 580-585 nm excitation), and stabilizers

packaging:
250 reactions* = 2 X 1.25 mL tubes
1250 reactions* = 10 X 1.25 mL tubes
5000 reactions* = 1 X 50 mL tube
*number of reactions based on a 20uL volume

Quality

Kit components are free of contaminating DNase and RNase. KiCqStart® SYBR® Green qPCR ReadyMix, Low ROX is functionally tested in qPCR. Kinetic analysis must demonstrate linear resolution over six orders of dynamic range (r2 > 0.995) and a PCR efficiency > 90%.

Other Notes

Storage Conditions:
KiCqStart SYBR Green qPCR ReadyMix is stable for 1 year when stored in a constant temperature freezer at -20°C, protected from light. For convenience, it may be stored unfrozen at +2 to +8°C for up to 6 months. After thawing, mix thoroughly before using. Repeated freezing and thawing of the product is not recommended. However, the product demonstrated no loss of performance after 20 freeze-thaw cycles or 2 months at +20°C.

Legal Information

Applied Biosystems is a registered trademark of Applera Corporation or its subsidiaries in the US and/or certain other countries

KiCqStart is a registered trademark of Qiagen Beverly Inc.

Mx3000P is a registered trademark of Agilent Technologies, Inc.

Mx3005P is a registered trademark of Agilent Technologies, Inc.

Mx4000 is a trademark of Agilent Technologies, Inc.

ROX is a trademark of Applera Corporation or its subsidiaries in the US and/or certain other countries

ReadyMix is a trademark of Sigma-Aldrich Co. LLC

Rotor-Gene is a registered trademark of Qiagen GmbH

SYBR is a registered trademark of Life Technologies

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

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Milli-Q® Water Purification Solutions
Protocols & Articles

Articles

Assay Optimization & Validation - PCR Technologies Guide

Use the table of contents on the right to navigate to other sections of A Technical Guide to PCR Technologies.
A Technical Guide to PCR Technologies
Keywords: AGE, Amplification, Buffers, Detection methods, Digestions, Electrophoresis, Gas chromatography, Gel electrophoresis, Gene expression, Melting, Nucleic acid annealing, Nucleic acid hybridization, PAGE, Polymerase chain reaction, Polymerase chain reaction - quantitative, Polymerization reactions, Polymorphisms, Purification, RNA purification, Separation, Size-exclusion chromatography, Transcription

Data Analysis - PCR Technologies Guide

Use the table of contents on the right to navigate to other sections of A Technical Guide to PCR Technologies.
A Technical Guide to PCR Technologies
Keywords: Amplification, Cancer, Capillary electrophoresis, Cell culture, Degradations, Electrophoresis, Gene expression, Growth factors, Indicators, Microarray Analysis, PAGE, Polymerase chain reaction, Polymerase chain reaction - quantitative, Purification, RNA purification, Sample preparations, Transcription

Polymerase Chain Reaction - PCR Technologies Guide

Use the table of contents on the right to navigate to other sections of A Technical Guide to PCR Technologies.
A Technical Guide to PCR Technologies
Keywords: Amplification, Cloning, Degradations, Detection methods, Electrophoresis, Evaporation, Gas chromatography, Gel electrophoresis, Indicators, Melting, Molecular biology, Nucleic acid annealing, Nucleic acid denaturation, PAGE, Polymerase chain reaction, Polymerase chain reaction - quantitative, Purification, Sequencing, Size-exclusion chromatography, Transcription

Quantitative PCR - PCR Technologies Guide

Use the table of contents on the right to navigate to other sections of A Technical Guide to PCR Technologies.
A Technical Guide to PCR Technologies
Keywords: Amplification, Buffers, Clinical, Clinical Chemistry, Detection methods, Electrophoresis, Enzyme activity, Gel electrophoresis, Gene expression, Melting, Methylations, Nucleic acid annealing, Nucleic acid denaturation, Nucleic acid hybridization, PAGE, Peptide synthesis, Polymerase chain reaction, Polymerase chain reaction - quantitative, Polymerization reactions, Size-exclusion chromatography, Transcription

Protocols

Imprint RIP | uRNA Quantitation | MicroRNA primers | MicroRNA Reverse Transcription

Sigma’s Imprint RNA Immunoprecipitation Kit was used to co-purify human argonaute 2 (Ago2)-associated RNAs from HeLa cells, essentially as instructed in the Technical Bulletin. To evaluate the differ...
Carol Kreader
Principal R&D Scientist
Keywords: Buffers, Cell disruption, Immunoprecipitation, Polymerase chain reaction - quantitative, RNA immunoprecipitation, Western blot

Kicqstart SYBR qPCR | Universal SYBR Green qPCR Protocol

KiCqStart SYBR Green qPCR ReadyMix is a 2X concentrated, ready-to-use reaction cocktail that contains all components, except primers and template, for real-time quantitative PCR (qPCR). This unique c...
Keywords: Amplification, Gene expression, Molecular probes, Nucleic acid annealing, Nucleic acid denaturation, Polymerase chain reaction, Polymerase chain reaction - quantitative, Size-exclusion chromatography, Titrations

Primer Optimization Using Temperature Gradient Protocol

One approach to assay optimization is to determine the optimum annealing temperature (Ta) of the primers by testing identical reactions containing a fixed primer concentration, across a range of anne...
Keywords: Amplification, Nucleic acid annealing, Nucleic acid denaturation, Polymerase chain reaction, Polymerase chain reaction - quantitative, Size-exclusion chromatography

SPUD Assay for Detection of Assay Inhibitors Protocol

The SPUD assay is one option for identification of inhibitors that may be present in RNA or DNA samples. The assay is particularly useful when a large number of samples are to be analyzed or when tar...
Keywords: Amplification, Nucleic acid denaturation, Polymerase chain reaction, Polymerase chain reaction - quantitative, Size-exclusion chromatography

SYBR® Green I Dye Quantitative PCR Protocol

Although quantitative PCR uses the same basic concept as traditional PCR, the reactions differ in that the amplicons are generally smaller and are detected indirectly using an additional dye or label...
Keywords: Gene expression, Nucleic acid annealing, Nucleic acid denaturation, Polymerase chain reaction, Polymerase chain reaction - quantitative, Size-exclusion chromatography

Universal SYBR Green Quantitative PCR Protocol

Technology Overview Assay Considerations Methods of Quantification Equipment & Supplies PCR Mix Selection Guide Protocol Troubleshooting Materials References
Keywords: AGE, Amplification, Buffers, Degradations, Electrophoresis, Enzyme activity, Gas chromatography, Gel electrophoresis, Gene expression, Genetic, Melting, Microarray Analysis, Nucleic acid annealing, Nucleic acid denaturation, Nucleic acid hybridization, Polymerase chain reaction, Polymerase chain reaction - quantitative, Polymorphisms, Purification, Sample preparations, Size-exclusion chromatography, Solvents, Titrations

qPCR Efficiency Determination Protocol

Once an assay has been optimized, it is important to verify the reaction efficiency. This information is important when reporting and comparing assays. In this example protocol, the assay efficiency ...
Keywords: Amplification, Gene expression, Nucleic acid denaturation, Polymerase chain reaction, Polymerase chain reaction - quantitative, Size-exclusion chromatography, Transcription

qPCR Gene Expression/Copy Number Analysis Using SYBR Green I Dye Detection Protocol

Measuring a target quantity relative to one or more stable reference genes using SYBR Green I dye detection is a common application of qPCR. Below is a standard protocol that can be adapted to specif...
Keywords: Nucleic acid annealing, Nucleic acid denaturation, Polymerase chain reaction, Polymerase chain reaction - quantitative, Size-exclusion chromatography, Transcription

qPCR Reference Gene Selection Protocol

Analysis of gene expression data requires a stable reference or loading control. This reference is usually one or more reference genes. Suitable reference genes are those which are unaffected by diff...
Keywords: Gene expression, Nucleic acid denaturation, Polymerase chain reaction, Polymerase chain reaction - quantitative, Size-exclusion chromatography, Transcription

Related Content

PCR Selection Guide

We offer a wide variety of PCR enzymes, master mixes, and PCR protocols to meet your experimental needs for routine PCR, qPCR, or RT-PCR. Our PCR Selection Guide features various filters to sort by, ...
Keywords: Genomics, Polymerase chain reaction, Polymerase chain reaction - quantitative

Peer-Reviewed Papers
15

References

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