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KEM0019 Sigma-Aldrich

T4 DNA Ligase (Rapid)

Ultra-pure enzyme for nucleic acid modifications

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Properties

grade   for molecular biology
assay   >99% (SDS-PAGE)
form   buffered aqueous solution
activity   300,000 U/mg
concentration   600,000 U/mL
shipped in   dry ice
storage temp.   −20°C

Description

Components

Supplied with:KEM0046B (2X Rapid Ligation Buffer)KEM0049B (10X T4 DNA Ligase Buffer)

Application

Suitable for:
• Restriction cloning
• TA cloning
• Adapter ligation
• NGS library construction
• Other applications requiring high efficiency ligation

Features and Benefits

• Ultra-purification process for ultimate enzyme performance
• Highest quality specifications for ultimate product consistency
• Undetectable DNA and nuclease contamination

Unit Definition

1 unit is defined as the amount of DNA Ligase required to join 50% of 100 ng of DNA fragments with cohesive termini in 50 μL 1X DNA Ligase Buffer following a 30 minute incubation at 23° C.

Physical form

Supplied in 10 mM Tris-HCl, 50 mM KCl, 1 mM DTT, 0.1 mM EDTA and 50% glycerol at pH 7.5 @ 25° C.

General description

T4 DNA Ligase catalyzes the formation of a phosphodiester bond between the terminal 5′ phosphate and a 3′ hydroxyl groups of duplex DNA or RNA. The enzyme efficiently joins blunt and cohesive ends and repairs single stranded nicks in duplex DNA, RNA or DNA/RNA hybrids.

Other Notes

Source of protein: A recombinant E. coli strain carrying the T4 DNA Ligase gene.

Unit size: 240,000 U

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis

Protocols & Articles

Articles

Nucleic Acid Modifying Enzyme Selection Chart (Ultra Pure)

DNA Polymerases synthesize DNA from nucleotides.  DNA polymerase is required for DNA replication, but also essential for many other activities in the cell, including genetic recombination, DNA repair...
Keywords: Amplification, Cloning, DNA replication, Genetic, Molecular biology, Peptide synthesis, Polymerase chain reaction, Purification, Recombination, Sequencing, Transcription, Whole genome amplification

Protocols

T4 DNA Ligase (Rapid) Protocol

T4 DNA Ligase catalyzes the formation of a phosphodiester bond between the terminal 5' phosphate and a 3' hydroxyl groups of duplex DNA or RNA. The enzyme efficiently joins blunt and cohesive ends an...
Keywords: DNA purification, Peptide synthesis, Polymerase chain reaction, Purification, transformation

Related Content

Synthetic Biology Resources

We are using our stregnths to help build the field of Synthetic Biology. Below is a collection of links to our favorite resources. In addtion to these resources, we also offer thousands of products f...
Keywords: Cell culture, Cloning, Gene expression, Genetic, Genetics, Metabolic Pathways, Metabolites, Molecular biology, Molecular biology techniques, Photosynthesis, Poisons, Polymerase chain reaction - quantitative, Transcription, Transfection

Peer-Reviewed Papers
15

References

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Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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