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L2164 Millipore

Anti-Luciferase antibody, Mouse monoclonal

clone LUC-1, purified from hybridoma cell culture

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Properties

Related Categories Alphabetical Index, Analytical and Industrial Enzymes, Antibodies, Biochemicals and Reagents, Enzymes, Inhibitors, and Substrates,
biological source   mouse
antibody form   purified immunoglobulin
antibody product type   primary antibodies
clone   LUC-1, monoclonal
form   buffered aqueous solution
mol wt   mol wt 60 kDa
concentration   ~2 mg/mL
application(s)   immunocytochemistry: 20-40 μg/mL using transfected 293Tcells expressing luciferase
  immunocytochemistry: suitable
  western blot: 2-4 μg/mL using whole extract of transfected 293T cells expressing luciferase
  western blot: suitable
conjugate   unconjugated
shipped in   dry ice
storage temp.   −20°C

Description

Application

Antibody suitable for immunoblotting and immunocytochemistry.

Preparation Note

Working Dilution: 1-2 μg/ml

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

General description

The mouse monoclonal Anti-Luciferase antibody recognizes the recombinant luciferase in transfected cells. This antibody provides user with an alternative detection method for luciferase. Since it directly detects the luciferase protein, it is not dependent on either the luciferase activity or the measeurement of rapid reaction kinetics. This also allows for the detection of the luciferase enzyme expression in situ with reproducibility between sample sets.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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