L5037 Sigma-Aldrich

Leptin from rat

≥97% (SDS-PAGE), recombinant, expressed in E. coli, lyophilized powder

Synonym: OB



Related Categories Cell Biology, Cell Signaling and Neuroscience, Cytokines Growth Factors and Hormones (Obesity), Cytokines, Growth Factors and Hormones, Gastrointestinal Peptides,
Quality Level   200
recombinant   expressed in E. coli
assay   ≥97% (SDS-PAGE)
form   lyophilized powder
storage temp.   −20°C
Gene Information   rat ... Lep(25608)



Leptin from rat has been used:
• to check the effect of intranasal nerve growth factor (NGF) on NGF activity in the spinal cord of Sprague-Dawley rats
• for validation of labelling for leptin-bearing cells of Sprague-Dawley rats
• to study the effect of leptin on STAT3 (signal transducer and activator of transcription 3) phosphorylation in neural population of Sprague-Dawley rats
• to study leptin response towards chemoreflex in nucleus of the solitary tract
• in rats, to check the effect of association between leptin and neuronal nitric oxide pathway on penicillin-induced epileptiform activity

Biochem/physiol Actions

In rodents with uncontrolled diabetes mellitus, leptin is crucial for normalizing hyperglycemia. In the digestive tract, it also participates in the absorption of dietary molecules via controlling transporters activity.

Leptin is a hormone produced primarily in adipocytes, although leptin mRNA has also been identified in placenta and fetal tissues, gastric tissue and liver. Its primary site of action appears to be on neurons in the hypothalamus that are involved in regulating energy balance, appetite, and body weight. Leptin increases the production of nitric oxide in endothelial cells and stimulates angiogenesis in vitro and in vivo.
Human and mouse leptin share ~84% sequence identity.

Physical form

Lyophilized from a 0.2 μm filtered solution with 4.5 mM NaHCO3.

Analysis Note

The biological activity is measured by its ability to induce proliferation of leptin-dependent human OB-R transfected murine BAF3 cells.

Safety & Documentation

Safety Information

Personal Protective Equipment 
NONH for all modes of transport
WGK Germany 


Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Protocols & Articles


Lipid Induced Insulin Resistance

Obesity is a well-established risk factor for the development of insulin resistance. Obesity is associated with the increased deposition of lipids in non-adipose tissue with subsequent decreases in i...
Linda Stephenson, Ph.D.
Biofiles v6 n4, 9
Keywords: Angiogenesis, Antivirals, Apoptosis, Biological processes, Cardiovascular, Catalog, Catalysis, Cell culture, Cell proliferation, Deposition, Diabetes, Events, Gene expression, Growth factors, Hormones, Inflammation, Metabolism, Metabolites, Obesity, Phosphorylations, Physiological Processes, Reductions, Transcription, Transduction, Type

Peer-Reviewed Papers


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