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L8511 Sigma-Aldrich

Leupeptin

synthetic, ≥85% (HPLC)

Synonym: Acetyl-Leu-Leu-Arg-al, Leupeptin hemisulfate salt, N-Acetyl-L-leucyl-L-leucyl-L-argininal hemisulfate salt

  • CAS Number 103476-89-7

  • Linear Formula C20H38N6O4 · 1/2H2SO4

  • Molecular Weight 475.59

  •  MDL number MFCD00037012

  •  PubChem Substance ID 24896498

  •  NACRES NA.77

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Properties

Related Categories Biochemicals and Reagents, Core Bioreagents, Enzyme Inhibitors, Enzymes, Inhibitors, and Substrates, Individual Protease Inhibitors,
Quality Level   200
biological source   synthetic
InChI Key   CIPMKIHUGVGQTG-VFFZMTJFSA-N
assay   ≥85% (HPLC)
solubility   H2O: 50 mg/mL
storage temp.   −20°C

Description

Biochem/physiol Actions

Inhibitor of serine and cysteine proteases. Inhibits plasmin, trypsin, papain, calpain, and cathepsin B. Does not inhibit pepsin, cathepsins A and D, thrombin, or α-chymotrypsin. Effective concentration 10-100 μM. There have been numerous studies using leupeptin to protect against hearing loss caused by acoustic overstimulation or the ototoxic antibiotic gentamicin. (Loss of cochlear hair cells is believed to be mediated by calpain.)

Analysis Note

Leupeptin gives multiple peaks on HPLC due to equilibria among three forms in solution. Purity determined using three main peaks. Majority of contaminating peptide is racemized leupeptin.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Single- & multi-channel pipettes from BRAND
Protocols & Articles
Peer-Reviewed Papers
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References

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