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M3168 Sigma-Aldrich

4-Methylumbelliferyl phosphate (4-MUP) Liquid Substrate System

liquid

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Description

General description

4-Methylumbelliferyl phosphate (4-MUP) is a substrate for alkaline phosphatase which forms the soluble fluorescent substance methylumbelliferone. 4-MUP Liquid Substrate System combines 4-MUP and buffer in a ready-to-use reagent.

Application

4-Methylumbelliferyl phosphate (4-MUP) Liquid Substrate System has been used as a fluorescent substrate for alkaline phosphate in enzyme linked immunosorbent assay (ELISA). It has also been used in secreted embryonic alkaline phosphatase (SEAP) activity assay.

Packaging

100 mL in poly bottle

Analysis Note

4-MUP fluorescence can be measured with excitation at 360 nm and emission at 440 nm. The fluorescent end product may also be observed using a UV light source.

Safety & Documentation

Safety Information

RIDADR 
UN 1760 8 / PGII
WGK Germany 
WGK 3
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles

Articles

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Peer-Reviewed Papers
15

References

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