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M4254 Sigma-Aldrich

5-Methylcytidine

≥99%

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Properties

Related Categories Biochemicals and Reagents, Nucleoside Analogs, Nucleosides, Nucleotides, Oligonucleotides More...
Quality Level   200
assay   ≥99%
form   powder
mp   212-215 °C (dec.) (lit.)
solubility   water: 25 mg/mL, clear, colorless
SMILES string   CC1=CN([C@@H]2O[C@H](CO)[C@@H](O)[C@H]2O)C(=O)N=C1N
InChI   1S/C10H15N3O5/c1-4-2-13(10(17)12-8(4)11)9-7(16)6(15)5(3-14)18-9/h2,5-7,9,14-16H,3H2,1H3,(H2,11,12,17)/t5-,6-,7-,9-/m1/s1
InChI key   ZAYHVCMSTBRABG-JXOAFFINSA-N

Description

General description

5-Methylcytidine is a component of RNA in Escherichia coli. It is present in the RNA of plant, animal and bacterial origin. It is one of the C derivative present in transfer RNA (tRNA).

Application

5-Methylcytidine has been used as a standard for deriving the calibration curve in reversed phase high performance liquid chromatography (RP-HPLC).

5-Methylcytidine is used in studies of DNA methylation processes (epigenetics).

Packaging

100 mg in poly bottle

25 mg in glass insert

Biochem/physiol Actions

5-Methylcytidine is an alkylated pyrimidine plays an important role enhancing the stacking in A- and B- helices of nucleic acid, results in increase of the melting temperature and improves thermal stability leading to structural stabilization.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 3
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Protocols & Articles

Articles

HPLC Analysis of Nucleosides on Ascentis® Express OH5 (HILIC Mode)

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Nucleosides on Ascentis® Express OH5 (HILIC Mode)
Keywords: Antivirals, Chromatography, High performance liquid chromatography, Metabolism

LC/MS Analysis of Nucleosides on Ascentis® Express F5

From our library of Articles, Sigma-Aldrich presents LC/MS Analysis of Nucleosides on Ascentis® Express F5
Keywords: Antibiotics, Antivirals, Chromatography, Dimroth rearrangement, Fungicides, Genetic, Herbicides, High performance liquid chromatography, Industries, Insecticides, Liquid chromatography mass spectrometry, Mass spectrometry, Rearrangements

UHPLC Analysis of Nucleosides on Purospher® STAR RP-18 endcapped

From our library of Articles, Sigma-Aldrich presents UHPLC Analysis of Nucleosides on Purospher® STAR RP-18 endcapped
Keywords: Antibiotics, Antivirals, Fungicides, Genetic, Herbicides, High performance liquid chromatography, Industries, Insecticides

Peer-Reviewed Papers
15

References

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