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M7522 Sigma-Aldrich

2-Mercaptoethanol

liquid, BioReagent, suitable for cell culture

Synonym: β-Mercaptoethanol, 2-Hydroxyethylmercaptan, BME, Thioethylene glycol

  • CAS Number 60-24-2

  • Linear Formula HSCH2CH2OH

  • Molecular Weight 78.13

  •  Beilstein/REAXYS Number 773648

  •  EC Number 200-464-6

  •  MDL number MFCD00004890

  •  PubChem Substance ID 24897168

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Properties

vapor density   2.69 (vs air)
vapor pressure   1 mmHg ( 20 °C)
InChI Key   DGVVWUTYPXICAM-UHFFFAOYSA-N
product line   BioReagent
form   liquid
expl. lim.   18 %
concentration   14.3 M (pure liquid)
refractive index   n20/D 1.500 (lit.)
bp   157 °C (lit.)
solubility   H2O: 1 mL/mL
density   1.114 g/mL at 25 °C (lit.)
suitability   suitable for cell culture

Description

Application

BME is suitable for reducing protein disulfide bonds prior to polyacrylamide gel electrophoresis and is usually included in a sample buffer for SDS-PAGE at a concentration of 5%. Cleaving intermolecular (between subunits) disulfide bonds allows the subunits of a protein to separate independently on SDS-PAGE. Cleaving intramolecular (within subunit) disulfide bonds allows the subunits to become completely denatured so that each peptide migrates according to its chain length with no influence due to secondary structure.

Other Notes

This product has been replaced by M3148-SIGMA | 2-Mercaptoethanol for molecular biology, for electrophoresis, suitable for cell culture, BioReagent, 99% (GC/titration)

Safety & Documentation

Safety Information

Signal word 
Danger
Target organs 
Heart, Liver
RIDADR 
UN 2966 6.1 / PGII
WGK Germany 
3
RTECS 
KL5600000

Documents

Certificate of Analysis

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Certificate of Origin

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Protocols & Articles

Articles

A Biologically-Inspired Electrochemical Half-Cell for Light-Driven Generation of Hydrogen

An economy based on hydrogen has long been touted as one of the most promising routes to a sustainable energy future. Hydrogen gas (H2) is a particularly clean source of energy that can be utilized e...
Rebecca A. Grimme1 and John H. Golbeck*1,2
Material Matters 2008, 3.4, 78.
Keywords: Absorption, Biochemistry, Catalysis, Deposition, Diffusion, Ligands, Mass spectrometry, Metabolism, Methods, Molecular biology, Nucleic acid denaturation, Photosynthesis, Recombination, Reductions, Semiconductor, Support, Type

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L-cysteine and L-cysteine equivalents in Cell Culture

Importance and uses of cysteine in serum-free eukaryotic, including hybridoma and Chinese Hamster Ovary (CHO) cell, cultures
Keywords: Cell culture, Culture media, Lipid peroxidation, Oxidations, Peroxidations, Reductions

Peer-Reviewed Papers
15

References

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