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M9956 Sigma-Aldrich

M9 Minimal Salts, 5X

Liquid microbial growth medium

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Properties

Related Categories Bacterial Culture Media, Microbial Media for Molecular Biology and Protein Expression, Molecular Biology, Other Media
Quality Level   GMP
grade   for molecular biology
sterility   sterile; 0.2 μm filtered
form   liquid
suitability   suitable for nonselective for Escherichia coli and Coliforms
storage temp.   room temp

Description

Components

15 g/L KH2PO4
64 g/L Na2HPO4
2.5 g/L NaCl
5.0 g/L NH4Cl

Features and Benefits

M9 minimal media provides:
• Filter-sterilized, ready-to-use format
• Convenient package size
• Standard formulation

Application

M9 Minimal Salts, 5X is suitable for non-selective cultivation of Escherichia coli strains for cloning, DNA plasmid production and production of recombinant proteins. It is also suitable for selective cultivation when appropriate antibiotics are added.

Suitable for non-selective cultivation of E. coli strains for cloning, DNA plasmid production and production of recombinant proteins. Also suitable for selective cultivation when appropriate antibiotics are added.

General description

M9 minimal media is a highly-referenced microbial growth medium used for the cultivation of E. coli. This nutrient-rich microbial broth contains peptides, amino acids, water-soluble vitamins, and carbohydrates.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
1

Documents

Certificate of Analysis


The Emprove® Program
Protocols & Articles

Articles

Molecular Biology Handbook

Molecular Biology is the science that aims to understand biological activity at the molecular level. These biological activities usually involve the plant or animal cell, and the nucleic acids and pr...
Keywords: Cell culture, Cell signaling, Cloning, Detection methods, Electrophoresis, Gel electrophoresis, Gene expression, Genetics, Molecular biology, Molecular biology techniques, Nucleic acid hybridization, Nucleic acid purification, Polymerase chain reaction - quantitative, Protein electrophoresis, Purification, Transfection, Western blot, transformation

Protocols

Initiating a Starter Culture

*This temperature is for standard E. coli. Some bacteria require different culture temperatures.
Keywords: Antibiotics

Peer-Reviewed Papers
15

References

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