• USA Home
  • MAK275 - High Sensitivity DHAP Fluorometric Assay Kit

EMAIL THIS PAGE TO A FRIEND
MAK275 Sigma-Aldrich

High Sensitivity DHAP Fluorometric Assay Kit

sufficient for 100 fluorometric tests

  •  NACRES NA.84

Purchase

Description

General description

Dihydroxyacetone phosphate (DHAP) is a metabolic intermediate involved in many pathways, including glycolysis, gluconeogenesis, glycerol metabolism, phosphatidic acid synthesis, fat metabolism and the Calvin cycle. Fructose-1,6-diphosphate is converted to DHAP and glyceraldehyde-3-phosphate (GAP) by aldolase. Both DHAP and GAP serve as the intracellular triose phosphate pool. DHAP can be further converted into GAP by triose phosphate isomerase (TPI). In humans, TPI deficiency is a rare autosomal disease. It causes hemolytic anemia, neurological diseases, and even death due to blockage of the glycolytic pathway and accumulation of DHAP in erythrocytes.

Dihydroxyacetone phosphate (DHAP) is a metabolic intermediate involved in many pathways, including glycolysis, gluconeogenesis, glycerol metabolism, phosphatidic acid synthesis, fat metabolism, and the Calvin cycle. Fructose-1,6-diphosphate is converted to DHAP and glyceraldehyde-3-phosphate (GAP) by aldolase. Both DHAP and GAP serve as the intracellular triose phosphate pool. DHAP can be further converted into GAP by Triose Phosphate Isomerase (TPI). In humans, TPI deficiency is a rare autosomal disease. It causes hemolytic anemia, neurological diseases, and even death due to blockage of the glycolytic pathway and accumulation of DHAP in erythrocytes.

Suitability

Suitable for use with serum, plasma, and other biological fluids, and tissue and cell culture samples.

Principle

The High Sensitivity Dihydroxyacetone Phosphate Assay Kit is suitable for measuring low levels of DHAP. In this assay, TPI converts DHAP to GAP producing a coupled enzyme reaction, which results in a fluorometric product (lex = 535 nm/lem = 587 nm), proportional to the amount of dihydroxyacetone phosphate present. This assay kit is simple and sensitive with the capability to detect to 0.5 mM of dihydroxyacetone phosphate in a variety of samples.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis (COA)

Please Enter a Lot Number
Protocols & Articles
Peer-Reviewed Papers
15

References

Related Products

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?