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MAK305 Sigma-Aldrich

CETP RP Activity Assay

(Supplied by Roar Biomedical, Inc.)

  •  NACRES NA.84

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Description

General description

Cholesteryl ester transfer protein (CETP) is present in normal human plasma and transfers neutral lipids from high density lipoproteins (HDL) to very low density lipoprotein (VLDL) and low density lipoprotein (LDL). CETP plays an important role in lipoprotein metabolism and influences the reverse cholesterol transport pathway.

Features and Benefits

Synthetic assay components support manipulation for MOA studies and SAR work. Unusually high signal-to-background with linearity. Assay components stable for up to 1 year. High DMSO tolerance.

Suitability

Suitable for high-throughput screening (HTS), mechanism of action (MOA) studies, and structure-activity relationship (SAR) work in CETP sources.

Principle

The CETP RP Activity Assay uses a proprietary substrate that enables the detection of CETP-mediated neutral lipid mass transfer. The method is useful for measuring CETP activity in recombinant protein (RP) or purified CETP samples and has a high DMSO tolerance. The transfer activity results in an increase in fluorescence intensity (λex = 465/λem = 535 nm). With recombinant CETP as the CETP source, the assay is linear over a wide range of CETP concentrations and provides very high signal-to-background values. Typical Z factor: ∼0.9

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis (COA)

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Protocols & Articles
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SMB00603 Cholesteryl Ester Transfer Protein (rCETP) human, recombinant, expressed in CHO cells, Supplied by Roar Biomedical, Inc.

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Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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