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MSKT0001 Sigma-Aldrich

SILuMAb K1 Pharmacokinetic Kit

Synonym: PK Kit

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Properties

suitability   suitable for mass spectrometry
storage temp.   2-8°C

Description

General description

The SILuMAb K1 Pharmacokinetic (PK) kit enables a robust, high-throughput assay for quantification of human IgG1 antibody in animal sera by LC-MS/MS. All critical reagents necessary to perform the assay are provided in the kit. The plate-based format avoids the high cost and potential LC column blockage associated with bead-based enrichment formats. Sample preparation with the SILuMAb PK kit can be performed in under 5 hours and consists of coating wells with high-specificity capture antibody, followed by immunoaffinity enrichment and rapid, in-plate trypsin digestion. The resulting digest is ready for injection, with no SPE or other cleanup required.

Legal Information

SILu is a trademark of Sigma-Aldrich Co. LLC

Safety & Documentation

Safety Information

RIDADR 
UN 3316 9
Protocols & Articles

Articles

A Universal Plate-Based Immunoaffinity LC-MS/MS Workflow for Preclinical Monoclonal Antibody Quantification

Monoclonal antibodies are the largest class of biotherapeutics approved for a variety of clinical indications, particularly in oncology and autoimmune diseases. During preclinical drug development, a...
Kevin Ray, Yue Lu, Pegah Jalili, Jeffrey Turner, Nicolas Caffarelli, and Tom Juehne
Keywords: Absorption, Clinical, Digestions, Diseases, High performance liquid chromatography, Liquid chromatography mass spectrometry, Mass spectrometry, Metabolism, Oncology

Development of a Simple and Rapid Digestion Protocol for Proteomics Sample Preparation

Reliable and reproducible results in mass spectrometry proteomics analyses are highly dependent on robust sample preparation. Sample preparation workflows are often cumbersome and generally involve c...
Zhiyun Cao, Amber Henry, Judy Boland, Nicolas Caffarelli, Jeffrey Turner, Kevin Ray
Keywords: Alkylations, Cell disruption, Detergents, Digestions, Mass spectrometry, Nucleic acid denaturation, Proteomics, Reductions, Sample preparations

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