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MSQC9 Sigma-Aldrich

SILuMAb Infliximab Stable-Isotope Labeled Monoclonal Antibody

recombinant, expressed in CHO cells

Synonym: SIL Infliximab

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Properties

Related Categories Antibody Characterization and Analysis, Biochemicals and Reagents, Mass Spectrometry, Molecular Biology, Peptide and Protein Standards for Mass Spectrometry Analysis,
recombinant   expressed in CHO cells
antibody product type   primary antibodies
assay   ≥90% (SDS-PAGE)
packaging   vial of 100 μg
shipped in   wet ice
storage temp.   −20°C

Description

Analysis Note

Quantitative
MRM settings provided (xls)

Preparation Note

Produced utilizing enriched media containing stable isotope labeled amino acids are 13C6, 15N4-labeled Arginine and 13C6, 15N2-labeled Lysine.

SILuMab Infliximab Stable-Isotope Labeled Monoclonal Antibody is designed to be used as a internal standard for analysis of Infliximab in human serum.

Reconstitution

SILuMab Infliximab recovery is maximized when 0.1% formic acid is used for reconstitution of the lyophilized product.Reconstitution with other solvents may reduce recovery. Do not freeze after reconstitution.

• Briefly centrifuge the vial at ~10,000 × g to collect the product at the bottom of the vial.
• Add 500 μL of ultrapure water containing 0.1% formic acid to the vial.
• Mix the contents by gently inverting the vial a minimum of 5 times.
• Allow the vial to stand at room temperature for at least 15 minutes and repeat mixing by inversion.

Physical form

Each vial of SILuMab Infliximab Stable-Isotope Labeled Monoclonal Antibody contains the labeled antibody in a lyophilized form containing phosphate buffered saline.

Application

Poster: Quantification of Infliximab in Human Serum by LC-MS/MS Using A Full-Length Stable Isotope Labeled Internal Standard

General description

SILuMAb Infliximab Stable-Isotope Labeled Monoclonal Antibody (MSQC9) is a recombinant, stable isotope-labeled, monoclonal antibody which incorporates [13C6, 15N4]-Arginine and [13C6, 15N2]-Lysine. Expressed in CHO cells, it is designed to be used as an internal standard for the quantitative mass spectrometry analysis of Infliximab in human serum. Infliximab is a chimeric monoclonal antibody biologic drug that works against tumor necrosis factor alpha (TNF-a) and is used to treat autoimmune diseases.
Infliximab has been approved for the treatment of Crohn′s disease, ulcerative colitis, psoriasis, psoriatic arthritis, ankylosing spondylitis, and rheumatoid arthritis. SILuMAb Infliximab Stable-Isotope Labeled Monoclonal Antibody is for research use only and is not intended for diagnostic or therapeutic use.

Legal Information

SILu is a trademark of Sigma-Aldrich Co. LLC

This product is licensed under U.S. Patent No. 7,396,688 and foreign counterparts from E. I. du Pont de Nemours and Company. The purchase of this product conveys to the buyer the nontransferable right to use the purchased amount of the product for research and development only, including services for a third party for consideration. The buyer cannot sell or otherwise transfer this product, its components or materials made using this product or its components to a third party. Information about licenses for excluded uses is available from: E. I. du Pont de Nemours and Company; Attn: Associate Director, Commercial Development; DuPont Experimental Station E268; 200 Powdermill Rd.; Wilmington, DE 19803; 1-877-881-9787 (voice), 1-302-695-1437 (fax), licensing@dupont.com.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis

Protocols & Articles

Articles

Development of Simple and Rapid Workflows for Quantitation of Infliximab and Adalimumab in Human Serum by LC-MS/MS

There is a growing demand for reliable LC-MS/MS assays to support quantitation of serum levels of infliximab and adalimumab monoclonal antibodies, as clinical responses differ between patients due to...
Kevin Ray, Yue Lu, and Pegah R. Jalili
MilliporeSigma, St. Louis, MO
Keywords: Centrifugation, Clinical, Digestions, High performance liquid chromatography, Liquid chromatography mass spectrometry, Mass spectrometry, Sample preparations

Quantification of Infliximab in Human Serum by LC-MS/MS

Infliximab, a chimeric monoclonal antibody, is used to treat rheumatoid arthritis, psoriatic arthritis, Crohn’s disease, and other autoimmune diseases. Clinical responses are different among patients...
Kevin Ray and Pegah R. Jalili
MilliporeSigma, St. Louis, MO
Keywords: Clinical, Digestions, Diseases, Glycosylations, High performance liquid chromatography, Liquid chromatography mass spectrometry, Mass spectrometry, Size-exclusion chromatography

UHPLC Analyses of Reduced Infliximab on BIOshell™ IgG 1000 Ã… C4 and BIOshell A400 Protein C4

From our library of Articles, Sigma-Aldrich presents UHPLC Analyses of Reduced Infliximab on BIOshell™ IgG 1000 Ã… C4 and BIOshell A400 Protein C4
Keywords: High performance liquid chromatography, Pharmaceutical, Reductions

UHPLC Analysis of Intact Infliximab on BIOshell™ IgG 1000 Ã… C4

From our library of Articles, Sigma-Aldrich presents UHPLC Analysis of Intact Infliximab on BIOshell™ IgG 1000 Ã… C4
Keywords: Diseases, Pharmaceutical

Related Content

Stable Isotope-labeled MS Internal Standards

What happens when you combine state-of-the-art technology with scientific prowess? Full-length protein standards with the highest purity and isotopic incorporation in the industry. Stable isotope-lab...
Keywords: Atomic absorption spectroscopy, Buffers, Digestions, Mass spectrometry, PAGE, Phosphorylations

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