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MTOX1016 Sigma

OATP1B3 Knockout HepaRG Cells

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Properties

Related Categories ADME/Tox Products, Cell Biology, Cell Line Models, Liver Drug Transporter/Toxicity Models
biological source   human female liver (hepatocarcinoma and hepatitis C tumor)
OMIM accession no.   605495
storage temp.   −196°C
Gene Information   human ... OATP1B3(28234)

Description

Application

See technical bulletin for detailed protocols

Legal Information

These products are covered by the License Agreement as described in Exhibit 1, 2 and 3, in the technical bulletin.

HepaRG is a trademark of BioPredic International company

Quality

Tested for Mycoplasma, sterility, post-freeze viability, short terminal repeat (STR) analysis for cell line identification, cytochrome oxidase I (COI) analysis for cell line species confirmation.

Features and Benefits

Zinc finger nucleases (ZFN) mediated Knock-out of SLCO1B3 (MRP3) gene.

• The frame-shift mutation of SLCO1B3 gene was confirmed by fragment length analysis and DNA sequencing.
• Loss of functionality was confirmed by loss of transport of selective substrates in sandwich culture assay.

General description

HepaRG is a human hepatoma cell line. The cells possess a pseudodiploid karyotype and have been characterized as an oval ductular bipotent hepatic cell line as they have the ability to differentiate into both biliary and hepatocyte lineages in the presence of DMSO. HepaRG OATP1B3 knockout cells express the major xenobiotic sensors (PXR, CAR and AhR), drug transporters, phase I and II drug metabolizing enzymes as well as key hepatic transcription factors involved in stress response pathways.

Safety & Documentation

Safety Information

RIDADR 
UN 3245 9
WGK Germany 
3

Documents

Certificate of Analysis


Milli-Q® Water Purification Solutions
Protocols & Articles

Articles

The Role of Liver Transporters in Drug-Drug Interactions

Oral drug delivery involves dissolution in the small intestine and absorption across the enterocyte barrier into the portal vein followed by subsequent delivery through the liver into the systemic ci...
David C. Thompson, Ph.D, R&D Manager, Sigma® Life Science and Michael D. Mitchell, Product Manager, Sigma® Life Science
Biofiles Vol. 8, No. 12
Keywords: Absorption, Cancer, Clinical, Eliminations, Genetic, Metabolic Pathways, Metabolism, Pharmaceutical, Polymorphisms

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