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N1142 Sigma-Aldrich

Neomycin solution

with 10 mg/mL neomycin in 0.9% NaCl, sterile-filtered, BioReagent, suitable for cell culture

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Properties

Related Categories Aminoglycosides, Antibacterial, Antibiotics, Antibiotics A to Z, Antibiotics N-S,
biological source   Streptomyces fradiae
sterility   sterile-filtered
product line   BioReagent
form   liquid
potency   8-12 mg per mL
packaging   pkg of 20ML
concentration   10 mg/mL in 0.9% NaCl (neomycin)
impurities   endotoxin, tested
color   clear
suitability   suitable for cell culture
Mode of action   protein synthesis | interferes
UniProt accession no.   O14746
antibiotic activity spectrum   Gram-negative bacteria
  Gram-positive bacteria
storage temp.   2-8°C
Gene Information   human ... TERT(7015)

Description

General description

Chemical structure: aminoglycoside

Biochem/physiol Actions

Neomycin Trisulfate is produced by Streptomyces containing a minimum of 85% neomycin B. It has been used as a selection agent for prokaryotic cells transformed using the neo selectable marker gene, and to study cytototoxic side effects of antibiotics, platelet-derived growth factor responses in certain fibroblasts, and extraction of Nuclear Phosphatidylinositol 4,5-Bisphosphate-Interacting Proteins. This product is recommended for use in cell culture applications at 5 mL/L.

Caution

This solution is formulated to contain 10 mg/mL neomycin in 0.9% sodium chloride. It is stable at 37 °C for 5 days and should be otherwise stored at 2-8°C.

Safety & Documentation

Safety Information

Symbol 
GHS08  GHS08
Signal word 
Danger
Hazard statements 
Precautionary statements 
RIDADR 
NONH for all modes of transport
WGK Germany 
2

Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Protocols & Articles

Articles

Antibiotic Kill Curve

Antibiotic kill curve is a dose response experiment in which mammalian cells are subjected to increasing amounts of selection antibiotic to determine the minimum concentration of an antibiotic that c...
Keywords: Antibiotics, Cell culture, Evaporation, Transfection

Related Content

Antibiotics Selection Guide

The Antibiotic Selection Guide enables researchers to spend more time on their research discoveries and less time seeking established antibiotic information. Link to hundreds of antibiotics for conta...
Keywords: Antibiotics, Cell biology, Cell culture, Genetic, High performance liquid chromatography, Reversed-phase chromatography

Peer-Reviewed Papers
15

References

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