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N2765 Sigma-Aldrich

4-Nitrophenyl phosphate disodium salt hexahydrate

tablet

Synonym: p-Nitrophenyl phosphate disodium hexahydrate, Disodium 4-nitrophenyl phosphate hexahydrate, di-Sodium 4-nitrophenyl phosphate, pNPP disodium hexahydrate

  • CAS Number 333338-18-4

  • Linear Formula O2NC6H4OP(O)(ONa)2 · 6H2O

  • Molecular Weight 371.14

  •  Beilstein Registry Number 5324661

  •  EC Number 224-246-5

  •  MDL number MFCD00066288

  •  PubChem Substance ID 24897592

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Description

Application

Chromogenic substrate for the determination of acid and alkaline phosphatases. The reaction produces a soluble end product that is yellow in color. The reaction may be stopped with 3 N NaOH and read at 405 nm.

Quantity

Contains 20 mg substrate per tablet

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3
Protocols & Articles

Articles

Colorimetric Alkaline Phosphatase and Peroxidase Substrate Detection Systems

Nitroblue Tetrazolium (NBT) is used with the alkaline phosphatase substrate 5-Bromo- 4-Chloro-3-Indolyl Phosphate (BCIP) in western blotting and immunohistological staining procedures. These substrat...
BioFiles 2008, 3.4, 6.
Keywords: Enzyme-linked immunosorbent assay, Immunohistochemistry, Western blot

Peer-Reviewed Papers
15

References

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