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N3254 Sigma-Aldrich

4-Nitrophenyl phosphate di(tris) salt

phosphatase substrate

Synonym: 4-Nitrophenyl phosphate bis(tris) salt

  • CAS Number 68189-42-4

  • Linear Formula (C6 H4 N O6 P)( C4 H12 N O3)2

  • Molecular Weight 461.36

  •  Beilstein/REAXYS Number 1578142

  •  EC Number 269-198-6

  •  MDL number MFCD00037009

  •  PubChem Substance ID 24897633

  •  NACRES NA.83

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Properties

Related Categories Alkaline Phosphatase, Alphabetical Listing, Biochemicals and Reagents, Colorimetric, Detection,
Quality Level   100
assay   ≥97% (enzymatic)
form   powder
solubility   water: 50 mg/mL, clear, colorless to light yellow
storage temp.   −20°C
SMILES string   NC(CO)(CO)CO.NC(CO)(CO)CO.OP(O)(=O)Oc1ccc(cc1)[N+]([O-])=O
InChI   1S/C6H6NO6P.2C4H11NO3/c8-7(9)5-1-3-6(4-2-5)13-14(10,11)12;2*5-4(1-6,2-7)3-8/h1-4H,(H2,10,11,12);2*6-8H,1-3,5H2
InChI key   XXAXKCWOTRABOW-UHFFFAOYSA-N
Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 3
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Protocols & Articles

Articles

Colorimetric Alkaline Phosphatase and Peroxidase Substrate Detection Systems

Nitroblue Tetrazolium (NBT) is used with the alkaline phosphatase substrate 5-Bromo- 4-Chloro-3-Indolyl Phosphate (BCIP) in western blotting and immunohistological staining procedures. These substrat...
BioFiles 2008, 3.4, 6.
Keywords: Enzyme-linked immunosorbent assay, Immunohistochemistry, Western blot

Peer-Reviewed Papers
15

References

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