O4126 Sigma-Aldrich

Oxaloacetic acid

≥97% (HPLC)

Synonym: 2-Oxosuccinic acid, Ketosuccinic acid, Oxalacetic acid, Oxobutanedioic acid

  • CAS Number 328-42-7

  • Linear Formula HOOCCH2COCOOH

  • Molecular Weight 132.07

  •  Beilstein Registry Number 1705475

  •  EC Number 206-329-8

  •  MDL number MFCD00002592

  •  PubChem Substance ID 24897993




1, 5, 25, 100 g in poly bottle


Oxaloacetic acid has been used as a substrate for measuring citrate synthase activity in cybrids and neuroblastoma cells. It has also been used for measuring malate dehydrogenase reactivation.

Biochem/physiol Actions

Oxaloacetic acid being an intermediate in the tri carboxylic cycle is central to metabolism. It is part of gluconeogenesis pathway. Mutation in pyruvate carboxylase leads to decreased production of oxaloacetate. It inhibits succinate dehydrogenase and is a key regulator of mitochondrial metabolism.

General description

Oxaloacetic acid is a dicarboxylic acid. It is an intermediate in the citric acid cycle. It is highly soluble in water and is present ubiquitously. It is produced in the mitochondria by the action of pyruvate carboxylase on pyruvate. Breakdown products of oxaloacetate includes malate, pyruvate and aspartic acid.

Safety & Documentation

Safety Information

GHS07  GHS07
Signal word 
Hazard statements 
Precautionary statements 
NONH for all modes of transport
WGK Germany 
Protocols & Articles


Citric Acid Cycle, TCA or Krebs Cycle

Acetyl-CoA enters a cycle whose discovery by Hans Krebs 70 years ago marked a milestone in biochemistry. The citric acid cycle is also termed the tricarboxylic acid cycle, TCA cycle or Krebs cycle (s...
BioFiles 2007, 2.6, 16.
Keywords: Addition reactions, Biochemistry, Catalysis, Citric Acid Cycle, Decarboxylations, Dehydration reaction, Hydration reaction, Metabolites, Oxidations, Phosphorylations

Fatty Acid Synthesis and Metabolism in Cancer Cells

Proliferatively active cells require fatty acids for functions such as membrane generation, protein modification, and bioenergetic requirements. These fatty acids are derived either from dietary sour...
Keywords: Apoptosis, Biofiles, Cancer, Carboxylations, Catalysis, Citric Acid Cycle, Gene expression, Glycolysis, Metabolism, Oxidations, PAGE, Phosphorylations

Glutamine Metabolism is Dysregulated in Many Cancer Cells

Proliferatively active cells require both a source of carbon and of nitrogen for the synthesis of macromolecules. Although a large proportion of tumor cells utilize aerobic glycolysis and shunt metab...
Keywords: Aerobic, Biofiles, Cancer, Citric Acid Cycle, Gene expression, Glycolysis, Metabolism, Metabolites, PAGE, Phosphorylations, Support, Transcription

Monosaccharide Biosynthesis

Each monosaccharide has its own interesting metabolism. The pathway by which a monosaccharide is made, is not the reverse of its degradation pathway (see Figure 4).
BioFiles 2007, 2.6, 21.
Keywords: Addition reactions, Carboxylations, Catalysis, Condensations, Decarboxylations, Degradations, Metabolic Pathways, Metabolism, Metabolites, Phosphorylations, Reductions


Enzymatic Assay of Malic Dehydrogenase (EC

The continuous spectrophotometric rate determination (A340, Light path = 1 cm) is based on the following reaction:
Keywords: Extinction coefficient

Peer-Reviewed Papers


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